沼泽红假单胞菌生物转化丹参醇提物转化酶初步定域

Preliminary localization of Rhodopseudomonas palustris tanshinol extract convertase

目的比较丹参醇提物经沼泽红假单胞菌以及其各细胞部位转化后化学成分的变化,对其转化酶在细胞部位水平上进行初步定域。方法利用沼泽红假单胞菌各细胞部位分别对丹参醇提物进行生物转化,高效液相色谱法(HPLC)分析转化前后主要化学成分的变化,用主成分分析法(PCA)对转化后变化程度进行评估。结果经不同细胞部位转化后,丹参醇提物的化学成分均发生变化。与对照比较,经细胞质、细胞膜化转后化学成分改变最大,表明细胞质、细胞膜含转化酶更多。结论沼泽红假单胞菌对丹参醇提物转化酶主要分布于细胞质、细胞膜上。

Objective To compare the differences in chemical compositions of tanshinol extract of Salvia milti- orrhizha （S. miltiorrhizha） bunge following conversion by Rhodopseudomonas palustris （R. palustris） and the individual cell organ, and to determine cellular location of tanshinol extract convertase. Methods We performed bioconversion of the tanshinol extract using different cellular organs of R.palustris. High-performance liquid chromatography was em- ployed to assess the changes of major chemical compositions before and after the bioconversion. We next applied principal component analysis （PCA） to evaluate the magnitude of variation in the chemical compositions. Results Following conversion by different cellular organs, there was significant change in all chemical components in the tan- shinol extract of S. miltiorrhizha bunge. Compared with control group, transformation using the cytoplasm and cellular membrane resulted in the most significant changes in the chemical components in tanshinol extract, suggesting that these two cellular organs harbored the most abundant convertase. Conclusion The convertase of tanshinol extract of S. miltiorrhizha bunge is mainly distributed in the cytoplasm and cellular membrane of R. palustris.