摘要
嫁接是提高黄瓜对根结线虫抗性的有效手段。本试验以对南方根结线虫抗性不同的黄瓜嫁接砧木‘云南黑籽南瓜'(低感)和‘甜砧'(高感),以及‘新泰密刺'黄瓜为试材,采用人工接种方法,比较研究了线虫侵染后三者的生理生化反应。结果表明,根结线虫侵染后,三者的叶片和根系中活性氧代谢、苯丙烷类代谢和病程相关蛋白活性均升高;在整个侵染过程中,超氧阴离子生成速率、过氧化氢含量、总酚含量和苯丙氨酸解氨酶等其他相关酶活性均先增高后降低,而木质素含量缓慢升高;三份材料中,以低感砧木‘云南黑籽南瓜'的物质含量和酶活性水平最高,升高幅度也最大。
Grafting is an effective way to enhance the resistance of cucumber to knot nematode. The study compared thephysiological and biochemical metabolism among the seedlings of nematode low sensitive cucumber rootstock variety‘Figleaf gourd’, highly sensitive ‘Tianzhen’ and cucumber ‘Xintaimici’ after artificial inoculated with Meloidogyneincognita.The results showed that after inoculated with Meloidogyne incognita, both roots and leaves of the three materialshad higher reactive as the metabolism of active oxygen, phenylpropanoid metabolism and pathogenesis-related proteinmetabolism. The generation rate of reactive oxygen, the content of hydrogen peroxide, total phenolic, phenylalanineammonia-lyase activity and other enzyme activities increased firstly and then decreased, but the content of lignin increasedslowly, and the ‘Figleaf gourd’ had the highest content, activity and growth.
出处
《山东农业大学学报(自然科学版)》
CSCD
北大核心
2014年第4期522-528,共7页
Journal of Shandong Agricultural University:Natural Science Edition
基金
国家"十二五"科技支撑计划课题(2011BAD12B04)
山东省科技攻关计划(2010GNC10939)
山东省自然科学基金(ZR2010CM016)
关键词
黄瓜
砧木
南方根结线虫
生理生化反应
Cucumber
rootstock
Meloidogyne incognita
physiological and biochemical reaction