耐盐差异性不同棉花品种的抗氧化酶活性及SNP/InDel分析

Antioxidant enzyme activities and SNP /InDel analysis of cotton varieties differring in salt tolerance

为探讨亲缘关系较近的耐盐棉花品种中棉所35与盐敏感棉花品种中棉所12耐盐差异性,用100mmol/L、200 mmol/L、300 mmol/L及400 mmol/L NaCl处理棉花幼苗,调查这2个棉花品种在盐胁迫下过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)等抗氧化酶活性的变化以及对中棉所35和中棉所12的SNP/InDel(单核苷酸多态性/碱基缺失或插入)进行了初步鉴定。结果显示,相比于盐敏感品种中棉所12,耐盐品种中棉所35的SOD活性随盐浓度上升变化较小,表现出较高的稳定性。盐敏感材料中棉所12的CAT及POD活性的变化趋势与中棉所35基本保持一致,在一定盐浓度内,随着盐浓度的上升而上升,超过一定的盐浓度后活性下降;盐敏感品种中棉所12的CAT及POD活性整体上低于耐盐品种中棉所35。此外,发现亲缘关系很近而耐盐水平差异显著的中棉所35和中棉所12存在点突变和插入/缺失,说明这些SNP/InDel可能对耐盐性状有重要的调控功能。

In order to explore the mechanism underlying the salt tolerance of two cotton varieties with close genetic rela-tionship, CCRI 35（salt-tolerant） and CCRI 12（salt-sensitive） were treated with 100 mmol/L, 200 mmol/L, 300 mmol/L, and 400 mmol/L NaCl respectively, and the activities of the antioxidant enzymes, including catalase （CAT）, peroxidase （POD） and superoxide dismutase （SOD） were measured. The results showed that the SOD activity of CCRI 35 was independent of salt con-centration, suggestive of high level of stability. The two cotton varieties had similar variation tendency of CAT and POD activities increased with the increase of salt level and decreased after wards. The activities of CAT and POD were lower in salt-sensitive va-riety CCRI 12 than those in salt-resistant variety CCRI 35. In addition, single nucleotide polymorphisms （ SNPs） and insertion/ deletion （InDel） between CCRI 35 and CCRI 12 were detected, indicating that the SNPs/ InDel might play an important role in controlling salt tolerance in cotton.