摘要
为了找到适用于转录组测序要求的大白菜小孢子总RNA提取方法,以出胚率较高的吉红82为试材,以热激诱导处理后的小孢子为研究对象,比较分析了TRIzol法、改良CTAB法及超纯试剂盒法3种方法的RNA提取效果。结果表明:TRIzol法(磨样)和改良CTAB法提取的RNA浓度较高,但完整性较差;超纯RNA提取试剂盒法提取的RNA质量最高,且完整性好,能够满足转录组测序的要求。
In order to find out micro-spore extracting method suitable for transcriptome sequencing,this study took Chinese cabbage‘Jihong 82’as experimental material and micro-spore induced with heat shock as research object.This study compared and analyzed the RNA extraction effects by 3 different methods:TRIzol method,improved CTAB method and ultrapure kit method.The results showed that RNA concentrations extracted by TRIzol method and improved CTAB method were higher,but the RNA integrity was lower.Quality of RNA extracted by ultrapure kit method was the highest,and the RNA inegrity was also good.Therefore,RNA obtained by this method was suitable for satisfying the requirement of transcriptome sequencing.
出处
《中国蔬菜》
北大核心
2014年第11期15-19,共5页
China Vegetables
关键词
大白菜
小孢子
RNA提取
转录组测序
Chinese cabbage
Micro-spore
RNA extraction
Transcriptome sequencing
