人参提取物达玛烷苷元对人体运动后肌糖原恢复的影响及机制研究

Effect of dammarane sapogenin extracted from ginseng on muscle glycogen recovery after aerobic exercise in human

目的:给受试者服用不同浓度的人参提取物达玛烷苷元(Dammarane Sapogenin,DS),探究其对有氧运动后肌糖原恢复的影响及作用机制。方法:本研究采用双盲实验设计,选取9名受试者随机分为安慰剂组(placebo group)、服用DS 240 mg/day组(DS240)、服用DS 480mg/day组(DS480)进行试验。受试者持续服用DS 4周后,进行一次性60分钟、强度为75%VO2max的蹬功率自行车运动,运动后立刻给予高糖饮食。于运动后即刻及运动后3小时,分别进行肌肉活检,测定AKT、GS磷酸化及蛋白质表达;同时,运动后3小时内每隔30分钟取静脉血测定血糖浓度、胰岛素浓度。结果:DS240组较对照组显著提升运动后肌糖原储存速率(P<0.05);运动后随着时间延长,DS240组的血糖浓度显著低于安慰剂组(P<0.05),但各组胰岛素浓度无显著性差异;运动后DS240组显著提高运动后AKT磷酸化水平、降低肌糖原合成酶磷酸化、提高肌糖原合成酶的蛋白质表达(P<0.05)。结论:长期补充低剂量达玛烷苷元可能提高人体运动后肌糖原储存速率,其可能机制与提升胰岛素作用及血糖吸收有关;研究结果推测达玛烷苷元可以增加胰岛素敏感度,因而可以将达玛烷苷元作为补剂用以运动竞赛或训练后加速肌糖原恢复。

Objective ： To investigate the influence of Dammarane Sapogenin make on glycogen＇ s recovery by making subjects ingest different doses of DS. Methods ：This study adopted double blind counter-balancing study and 9 subjects were divided random into placebo group, DS240 （DS240 mg/day）, and DS480（DS 480 mg/day）. The subjects did an acute 60- mins cycling exercise （75% VO2max） after having taken DS for 4 weeks and subjects would be given high carbohydrate meal immediately after exercise. Muscle biopsy, AKT phosphorylation, GS phosphorylation and protein expression should be performed immediately and 3 -h after exercise. At the same time, Blood Glucose and Blood insulin should be tested every 30 mins in 3 hours after exercise. Results：： Compared with the placebo group, the muscle glycogen storage rates of DS240 was greater. After aerobic exercise, blood sugar concentration of DS240 was significantly lower than those of placebo group, but the difference of the insulin concentration was not significant. AKT phosphorylation of DS240 group was greater than those of placebo group. After the supplement of DS, phospho - glycogen syn-thase was decreased and the protein expression of muscle glycogen synthase was increased significantly. Conclusions：Taking lowdose of DS for a long time may improve muscle glycogen storage rates and it shows that DS may increase the sensitivity of insulin, which can make DS be supplements and can be used in sports competitions and the recovery of glycogen after exercise.