针刺刺激量对寒凝证类痛经模型大鼠疼痛反应、子宫前列腺素含量的影响

Effects of Acupuncture Stimulating Quantity on Pain Reactions and Content of Uterine Prostaglandin in Dysmenorrheal Rats with Cold Stagnation Syndrome

目的:观察不同针刺刺激量对寒凝证类痛经大鼠扭体反应和子宫前列腺素E2(PGE2)、前列腺素F2α(PGF2α)含量的影响,初步探讨针刺的量效关系。方法:将雌性大鼠40只,随机分为正常组、模型组、刺激量A组(粗针进针深,针刺时间长)、刺激量B组(细针进针浅,针刺时间短)。除正常组外,模型组、刺激量A组和刺激量B组采用全身冷冻法结合苯甲酸雌二醇注射法造模。模型制备后,针刺治疗20 min,观察大鼠的扭体反应,并测定大鼠子宫组织内前列腺素E2(PGE2)、前列腺素F2α(PGF2α)含量。结果:1与正常组比较,模型组扭体潜伏期明显缩短(P<0.01),扭体次数及扭体评分明显增加(P<0.01)。与模型组比较,刺激量A组扭体潜伏期明显延长(P<0.05),扭体次数及扭体评分明显减少(P<0.01);刺激量B组扭体次数明显减少(P<0.01)、扭体评分明显减少(P<0.05)。2与正常组比较,模型组PGF2α含量、PGF2α/PGE2比值明显升高(P<0.01)。与模型组比较,刺激量A组PGF2α含量、PGF2α/PGE2比值明显降低(P<0.01);刺激量B组PGF2α含量明显降低(P<0.05),PGE2含量明显升高(P<0.01),PGF2α/PGE2比值明显降低(P<0.01)。结论:针刺刺激量与镇痛效应具有相关性。针刺可明显减轻大鼠的类痛经反应,可能的机制是通过调节子宫前列腺素E2(PGE2)、前列腺素F2α(PGF2α)的紊乱水平而缓解子宫痉挛性收缩。

Objective：To observe the effects of different quantity of acupuncture stimulation on writhing response and uterine prostaglandin E2（PGE2）,prostaglandin F2α（PGF2α） contents in dysmenorrheal rats with cold stagnation syndrome,to study relationship between quantity and effect of acupuncture stimulating. Methods：Total 40 female rats were divided into 4 groups randamly,including：normal group,model group,A stimuli group,B stimuli group. Except for normal group,the models were established by general freezing method combined with estradiol benzoate injection molding. After model preparation,acupuncture treatment were given for 20 min,to observe the writhing response in rats,and to detect PGE2,PGF2αcontent in rat uterus tis sue. Results：1Compared with the normal group,the writhing latency of the model group was significantly shortened（P〈0.01）,the writhing times and writhing score increased significantly（P〈0.01）. Compared with the model group,writhing latency of A stimuli group significantly longthened（P〈0.05）,the writhing times and writhing score decreased significantly（P〈0.01）. B stimuli group,the writhing times decreased significantly（P〈0.05）,the writhing score decreased significantly（P〈0.01）. 2Compared with the normal group,PGF2αcontent,PGF2α/PGE2 of the model group increased significantly（P〈0.01）. Compared with the model group,PGF2αcontent,PGF2α/PGE2 of A stimuli group decreased significantly（P〈0.01）. In B stimuli group,PGF2αcontent decreased significantly（P〈0.05）,PGE2 content increased significantly（P〈0.01）,PGF2α/PGE2 decreased significantly（P〈0.01）. Conclusion：Acupuncture can obviously reduce pain reactions in dysmenorrheal rats,relieve spastic contraction of the uterus by regulating disorder level of uterine PGE2,PGF2α,which confirmed the correlation between acupuncture stimulating quantity and analgesic effect.