运动和药物干预对2型糖尿病大鼠骨髓瘦素及其受体蛋白表达的影响

Effects of Exercise Combined with Drug Intervention on Serum Makers and Protein Expression of Leptin and Leptin Receptor of Bone Metabolism in Type 2 Diabetic Rats

目的:探讨有氧耐力运动和药物(二甲双胍)干预对糖尿病大鼠血清骨代谢生化指标及骨髓瘦素、瘦素受体蛋白表达的影响。方法:以59只雄性SD大鼠为研究对象,随机分为5组,正常饮食对照组(NCG)、糖尿病对照组(DCG)、糖尿病运动组(ETG)、糖尿病药物组(DTG)和糖尿病药物运动组(ETDTG),除NCG组用普通饲料喂养外,其余各组均用高脂饲料喂养,通过6周高脂高糖膳食并注射链脲佐菌素建立糖尿病模型后,ETG组和ETDTG组进行为期6周的游泳训练,DTG组和ETDTG组按体重灌胃6周二甲双胍。各组大鼠于第12周结束时取样,检测糖代谢相关指标:血糖与胰岛素,血清骨代谢生化指标:血清碱性磷酸酶、血清磷离子、血清钙离子,骨髓分子生物学指标:瘦素、瘦素受体蛋白表达。结果:与NCG组相比,DMC组体重、空腹血糖、胰岛素水平显著升高(P<0.05,P<0.01,P<0.01),与DCG组相比ETG组、DTG组和ETDTG组空腹血糖、胰岛素有下降趋势,其中以ETDTG组的下降最为明显;与NCG组相比,DCG组血清碱性磷酸酶显著升高(P<0.01),血清钙离子、磷离子显著降低(P<0.01);ETDTG组与DCG组相比血清碱性磷酸酶显著降低(P<0.05),血清钙离子、磷离子显著升高(P<0.01,P<0.05);与NCG组相比,DCG组大鼠瘦素水平显著升高、瘦素受体水平显著降低(P<0.01,P<0.01),与DCG组相比,ETDTG组瘦素水平显著降低,瘦素受体水平显著升高(P<0.01,P<0.05)。结论:有氧运动训练和药物干预均可通过改善糖尿病大鼠骨髓瘦素抵抗来改善大鼠骨代谢紊乱,其中运动结合药物组作用效果更好。

Objective：To investigate the effects of a combined aerobic endurance exercise and drug （metformin） interven- tion on biochemical markers of serum bone metabolism in diabetic rats. Methods： A total of 59 male SD rats were used as research objects and were randomly divided into five groups：normal diet control （NC） group, diabetic control （DC）group, exercise-trained （ET） group, drug-treated （DT） group and exercise training plus drug treatment （ETDT） group. Apart from NC group of rats fed with normal food, other groups of rats were all fed on a high fat diet for six weeks with streptozotocin injection in building diabetes model. Rats in both ET group and ETDT group were girken six weeks of swimming training and rats in DT group and ETDT group were administered intragastrically with Met in body weight for six weeks after dia- betic model was established. At the end of 12 week, rats from each group were assigned for specimen harvest and undergo the detection of related indicators of carbohydrate metabolism which included plasma glucose concentration, glycosylated serum protein and serum insulin concentration, and serum makers of bone metabolism including osteocalcin and tartrate-re- sistant acid phosphatase levels. Results：Compared with NC group, diabetic control rats had significantly increased the levels of body weight, insunlin, fasting blood-glucose in DC Group （ P 〈 0.05, P 〈 0. 01 ）, and compared with those of DC group, a downward trend was obtained for the levels of body weight, insunlin, fasting blood-glucose in the ET group, DT group and ETDT group, and the decline was statistically significant for the ETDT group （P 〈 0. 01, P 〈 0.01, P 〈 0. 05 ） ;At the same time,in DCG group, compared with ETG group, DTG group and ETDTG fasting blood glucose, insulin downward trend, with the most obvious decline in ETDTG group;compared with the NCG group serum alkaline phosphatase signifi- cantly increased in DCG group （P 〈0. 01 ）, and serum calcium,phosphorus ions decreased significantly （P 〈 0. 01 ） ;ET- DTG group DCG group compared with serum alkaline phosphatase decreased significantly （ P 〈 0.05 ）, serum caicium, phosphorus ions were significantly higher （P 〈 0. 01, P 〈 0. 05 ） ;compared with the NCG group, DCG leptin levels were significantly higher in rats ,and leptin receptor levels were significantly lower （P 〈0. 01 ,P 〈 0. 01 ）. Compared with DCG groups, ETDTG group leptin significantly was lower and leptin receptor levels were significantly increased （P 〈 0. 01, P 〈 0. 05）. Conclusion： Both aerobic exercise training and drug therapy can improve disorder of bone metabolism in rats through changing the leptin level but aerobic exercise training combined drug treatment proved to be much more effective than either approach alone.