基于纳米氧化铜修饰的异戊巴比妥的新型电化学传感器的制备与应用

Preparation and Application of Novel Amobarbital Electrochemical Sensor Based on CuO Nanoparticles Modified Glassy Carbon

在纳米氧化铜修饰的玻碳电极表面电聚合一种能够快速检测尿液中异戊巴比妥（AMB）的分子印迹敏感膜,研究了该敏感膜的最佳成膜条件及最佳工作条件.通过扫描电子显微镜（SEM）、循环伏安（CV）和电化学交流阻抗法（EIS）研究了印迹膜的表面形貌及性能.电化学实验结果表明,纳米氧化铜能提高传感器对AMB的灵敏度.在最佳实验条件下,铁氰化钾分子探针的差分脉冲伏安（DPV）峰电流响应值与AMB的浓度在1.0×10-7-1.4×10-4mol/L范围内呈现良好的线性关系（线性相关系数R=0.9966）;检出限为2.1×10-9mol/L（S/N=3）.此印迹传感器可用于尿液中AMB的检测,加标回收率为94.00%-104.67%.

A novel sensor is developed for the detection of amobarbital in urines, which is based on an electropolymerized molecularly imprinted polymer （MIP） on the surface of the CuO nanoparticles modified glassy carbon electrode. The optimums film formed conditions and experimental conditions were explored. The surface feature and performance of the modified electrode were characterized by scanning electron microscope （SEM）, cyclic vohammetry（CV） and electrochemical impedance spectroscopy（EIS）. The results of electrochemical measurements indicated that CuO nanoparticles had good sensitization effected for the molecularly imprinted sensor and it exhibits good sensitivity and selectivity to the template molecule amobarbital. Under the optimal conditions, the relative redox peak currents of hexacyanoferrate were linear. The concentration of amobarbital ranged from 1.0×10-7 to 1.4×10-4 mol/L, with a linear correlation coefficient of 0. 9966. The detection limit was 2. 1 × 10-9 mol/L（ S/N = 3 ）. The prepared sensor was applied to the determination of amobarbital in urine samples with recovery ranging from 94. 00% to 104. 67%.