希瓦氏菌介导制备还原氧化石墨烯薄膜及其增强的胞外电子传递

Shewanella-mediated Synthesis of Reduced Graphene Oxide Film for Enhanced Extracellular Electron Transfer

采用单室、三电极体系,通过希瓦氏菌(Shewanella)还原氧化石墨烯(GO)膜,制备了石墨烯(rGO)薄膜,并通过X射线衍射(XRD)和拉曼光谱(Raman)等手段表征了GO还原前后的结构和性质,证明了Shewanella菌可以有效将GO膜还原为rGO.以该生物还原得到的rGO薄膜为工作电极,可以有效改善Shewanella菌的胞外电子转移(EET).与空白的氧化铟锡(ITO)电极相比,rGO电极的阳极输出电流增大了13%,电量增加90%.该Shewanella还原法是一种温和制备rGO膜的方法,制得的rGO膜改善了微生物的EET效率.

A novel route was developed to prepare reduced graphene oxide （rGO） films through reduction of graphene oxide（GO） by Shewanella loihica PV4 （S. loihica PV-4） in a single-chamber, three-electrode system. Molecular structures of GO and rGO were characterized by X-ray diffraction and Raman spectroscopy. The results demonstrated that GO film was effectively reduced to rGO by S. loihica PV4. The obtained rGO film was used as a work electrode to improve the extracllular electron transfer（EET） of S. loihica PV4. Compared to ITO electrode, the anode current of rGO electrode with a film thickness of 111 nm increased by 13%, and the electric quantity increased by 90%. The Shewanella-mediated reducing method may offer a simple and mild route to prepare electrode material for high performance microbial fuel cell.