高产纤维素酶的米曲霉菌种的筛选和以甘蔗糖蜜为碳源对培养基的优化

Screening of a High Cellulase Producing Strain Aspergillus Oryzae and Medium Optimization by Using Cane Molasses as Carbon Source

目的:根据生物量和纤维水解圈从米曲霉40636,41478,M1,M2 4株菌中确定40636为实验菌种。方法:在单因素试验基础上,进行正交试验设计。确定米曲霉40636的液体培养基为糖蜜25%,硫酸铵0.75%,蛋白胨0.375%,酵母粉0.375%,KH2PO4添加量为0.27%,MgSO4添加量为0.105%,氯化钠添加量为0.12%。在此条件下,米曲霉菌体干重达到20.2940g/L。

Objective：Based on biomass estimates and hydrolysis spot diameters, Aspergillus Oryzae 40636 is as the experimental strain screened out of four different strains （40636,41478,M1 and M2）. Method：Based on the single factor experiment,the orthogonal experimental design is carried out. Result：A dry cell weight of 20.2940 g/L is obtained under the optimum conditions using a fluid medi-um of 25% sugar molasses,0.75% ammonium sulfate,0.375% peptone,0.375% yeast powder,0. 27% monopotassium phosphate,0.105% magnesium sulfate,and 0.12% sodium chloride.