ILs-HPLC同时测定黄柏中5种生物碱含量

ILs-HPLC simultanesous determination of five alkaloids in Phellodenddri Chinensis Cortex

建立以离子液体作反相高效液相色谱流动相添加剂测定黄柏中黄柏碱、木兰花碱、药根碱、巴马汀以及小檗碱等5种主要生物碱含量的方法。实验以C18反相色谱柱为分离柱,采用紫外检测方法,考察了提取溶剂、检测波长、咪唑类离子液体烷基链长度、离子液体溶液浓度等条件对提取分离和测定影响。研究所建立的ILs-HPLC法,简便、快速、重复性好,离子液体的加入能改善C18柱分离生物碱的色谱峰形,并提高了分离度,本法可用于黄柏中多种生物碱的同时含量测定。

A RP-HPLC method was established for simultaneous determination of phellodendrine hydrochloride （PH1）, magnoflorine hydrochloride （ MH）, jatrorrhizine hydrochloride （ JH）, palmatine hydrochloride （ PH2 ） and berberine hydrochloride （BH） in Phellodendri Chinensis Cortex by using ionic liquids as mobile phase additives. The separation was performed on a Kromasil C18 （4.6 mm × 250 mm,5 μm） coupled with ultraviolet （UV） detection. The effect of extraction solvent, detection wavelength, length of alkyl chain on different imidazolium ionic liquids and concentration of ionic liquids on the separation and determination of alkaloids were investigated. Ionic liquid, [ BMIm ] BF4, can obviously improve the resolution and peak shape. This ILs-HPLC method is simple, rapid, and reliable, which can be used for determination of alkaloids in Phellodenddri Chinensis Cortex.