艾纳香叶废渣乙酸乙酯部位对佐剂类风湿关节炎大鼠药理作用研究

Anti-arthritic and anti-oxidative effect of ethyl acetate fraction of Blumea balsamlfera residues in rat adjuvant-induced arthritis

目的:研究艾纳香叶废渣乙酸乙酯部位(BBE)对大鼠实验性佐剂类风湿关节炎的作用及其机制。方法:用弗氏完全佐剂建立佐剂型类风湿关节炎大鼠模型,用废渣乙酸乙酯部位不同剂量进行28 d的灌胃治疗处理,以体重变化、足肿胀度、关节炎指数、免疫器官增生以及病理切片等来评价治疗效果,并同时用比色法测定血清中的SOD,MDA,GSH,NO,OH·,ALP,AST,ALT,NAG,SA水平以及采用酶联免疫吸附实验(ELISA)测定血浆中IL-1,IL-6,TNF-α水平。结果:其乙酸乙酯部位能显著减轻AA大鼠关节肿胀和关节炎指数,能有效抑制关节滑膜增生,下调血清中MDA,NO,OH·,ALP,AST,ALT,NAG,SA,IL-1,IL-6,TNF-α水平,上调SOD,GSH水平。结论:艾纳香叶废渣乙酸乙酯部位对类风湿关节炎有一定的治疗作用。

Objective： To study the effect of ethyl acetate fraction of Blumea balsamifera （BBE） residue on treating rats of adjuvant arthritis （AA） and its mechanism. Method： The rats were immunized with the Freund＇s complete adjuvant （FCA）. After modeling, 28 days＇ treatment with BBE was performed. During the experimental process, rat mass, toe girth, arthritic index （AI）, proliferation of immune organs and pathological section were measured. After treatment, blood samples were collected through fossa orbitalis vein for detection of serum SOD, MDA, GSH, NO, OH · , ALP, AST, ALT, NAG and SA content using colorimetric method and IL- 1, IL-6, TNF-α content using ELISA method. Result： Administration with BBE （high dose） could significantly amehorate joint swelling and arthritis index, effectively inhibit synovial hyperplasia, down-regulate the levels of MDA, NO, OH · , ALP, AST, ALT, NAG, SA, IL-1, IL-6, TNF-α and up-regulate the SOD and GSH levels in serum. Conclusion： The results suggested BBE possesses substantial anti-arthritis and antioxidant activities.