摘要
为提高莲资源的综合利用水平,采用纤维素酶法提取荷叶多酚,以多酚得率为考察指标,通过单因素和正交试验,探讨纤维素酶含量、料液比、pH值、提取温度、提取时间、提取次数对荷叶多酚得率的影响。结果表明,纤维素酶法提取荷叶多酚的最佳条件为:纤维素酶含量0.5%、料液比1∶30、pH值4.8、提取温度50℃、提取时间45min、提取次数3次,在此条件下荷叶多酚得率最高,为2.699%。
In order to improve the comprehensive utilization level of lotus resource,lotus leaf was used to extract polyphenols with cellulase,and the effects of cellulase concentration,solid-liquid ratio,pH value,extraction temperature,extraction time and times on the extraction yield of polyphenols were studied,and the optimum extraction parameters were confirmed through the single factor and orthogonal experiments.The results showed that the optimum cellulase concentration was 0.5%,solid-liquid ratio was 1∶30 （m/V）,pH value was 4.8,extraction temperature was 50 ℃,extraction time was 45 min,extraction times was three.Under this optimum condition,the extraction yield of polyphenols was 2.6 9 9%.
出处
《河南农业科学》
CSCD
北大核心
2014年第9期177-180,共4页
Journal of Henan Agricultural Sciences
基金
湖北省科技计划项目(2012DCB04001)
特色果蔬质量安全控制湖北省重点实验室开放项目(2013K06)
湖北工程学院科研项目(z2013002)
关键词
荷叶
多酚
纤维素酶
提取
lotus leaf
polyphenols
cellulase
extraction
