全反式视黄醛水包油微乳体系的激光闪光光解研究

Study on laser flash photolysis of all-trans retinene in oil-in-water microemulsion

以十二烷基磺酸钠为表面活性剂,环己烷为油相,正丁醇为助表面活性剂制备了全反式视黄醛(All-trans retinene,ATRN)的水包油微乳体系,然后利用355 nm激光闪光光解技术,研究了微乳中ATRN的瞬态光化学性质。结果表明,ATRN经由355 nm激光激发,生成ATRN激发三重态(3ATRN*),3ATRN*的瞬态特征吸收波长为470 nm。在酸性环境中,3ATRN*能与二苯胺发生电子转移反应,生成ATRN中性自由基和二苯胺阳离子自由基。通过研究3ATRN*与生物分子和抗氧化剂之间的反应表明:3ATRN*反应活性不高,由此推断ATRN可能经由光动力TypeⅡ途径,造成生物体系的光毒性。

Oil-in-water microemulsion of all-trans retinene （ATRN） was synthesized in which SDS was used as surfactant, 1-butanol as cosurfactant, and cyclohexane as oil phase. Then 355 nm laser flash photolysis was used to investigate the transient photochemistry of ATRN in the microemulsion. The results showed that ATRN can be photoexcited by 355 nm laser light to produce the triplet state of ATRN （3ATRN＊） with 2max 470 nm. In acidic solution, 3ATRN＊ can react with diphenylamine （DPA） via electron transfer to produce transient products of ATRN neutral radical and DPA radical cation. The study on the reactivity of 3ATRN＊ with lysozyme, amino acids and antioxidants showed that 3ATRN＊ may not interact directly with biomolecules, and ATRN may be phototoxic via PDT Type II photosensitization process.