二氧化硅-氧化石墨烯复合物固相萃取-高效液相色谱法检测植物油中黄曲霉毒素B_1、B_2

Determination of Aflatoxin B_1 and Aflatoxin B_2 in Edible Oil by Using Graphene Oxide-SiO_2 as Soild Phase Extraction Coupled with HPLC

以二氧化硅-氧化石墨烯复合物为固相萃取材料,建立了植物油中黄曲霉毒素B1、B2的高效液相色谱（HPLC）检测方法。优化的条件为：复合材料的最佳用量为0.15 g,最佳萃取时间20 min,洗脱溶剂为乙腈,洗脱次数为2次。结果表明,在优化条件下,建立的二氧化硅-氧化石墨烯复合物固相萃取-高效液相色谱法对黄曲霉毒素B1、B2的检出限分别为0.17和0.05μg/L。将本方法应用于植物油实际样品的检测中,加标回收率在81.4%~105.3%之间,相对标准偏差为1.3%~8.6%。

Silica dioxide bound graphene oxide （GO-SiOz ） was applied as an effective adsorbent for determination and quantification of aflatoxin B1, B2 in edible oil by HPLC. The optimized conditions were GO-SiO2 0.15 g, extraction time 20 min, elution reagent acetonitrile, elution cycles two times. Results showed under the optimum conditions, the detection limits of afiatoxin B1 , B2 were 0.17 and 0.05 μg/L, respectively. The method was successfully applied to the detection of the actual edible oil, the spiked recoveries of aflatoxin B1 and B2 were 81.4%-105.3% and the relative standard deviations were 1.3%-8.6%.