Livin与caspase-3蛋白在胃腺癌组织中的表达及临床意义

Study on the Expression of Livin and Caspase-3 in Stomach Adenocarcinoma Tissue and Its Clinical Significance

目的研究生存蛋白(livin)与胱天蛋白酶3(caspase-3)在胃腺癌组织中的表达及其临床意义。方法选择2010年1月至2012年6月阜宁县人民医院经过病理诊断确诊为胃癌的蜡块标本114例作为胃癌组,另外取病理诊断为正常胃黏膜的蜡块标本46例作为对照组,应用免疫组织化学染色检测两组livin与caspase-3蛋白的表达情况,分析比较其表达与临床病理特征的相关性以及两者之间的相关性。结果 Livin在胃腺癌组织中的阳性表达率显著地高于正常胃黏膜组织(χ2=66.74,P<0.05),caspase-3在胃腺癌组织中阳性表达率低于正常胃黏膜组织(χ2=68.01,P<0.05)。不同临床分期、组织分化、肿瘤浸润深度以及淋巴转移与否的livin和caspase-3阳性率比较差异均有统计学意义(P<0.05)。Livin和caspase-3在胃腺癌组织表达呈负相关(rs=-0.57,P<0.05)。结论Livin和caspase-3在胃腺癌的表达呈负相关,提示livin可能通过抑制细胞凋亡来促进胃腺癌的发生、发展。

Objective To investigate the expression of Livin and Caspase-3 in stomach adenocarcinoma tissue and its clinical significance. Methods A total of 114 wax samples of stomach adenocarcinoma tissue from patients admitted in Funing People＇s Hospital from Jan. 2010 to Jun. 2012 were collected as the stomach adenocarcinoma gourp, another 46 wax samples of normal gastric mucosa tissue were collected as the control group. The expression of livin and Caspase-3 of the two groups was detected by staining, and the correlation between the expression levels of livin and Caspase-3 as well as the correlation between such expressions and the clinical pathological characteristics were analyzed. Results The positive expression rate of livin in the stomach adenocarcinoma tissue was higher than in the normal gastric mucosa tissue,with significant statistical difference （X^2 = 66.74,P 〈 0.05 ） ;the positive expression rate of Caspase-3 in the stomach adenocarcinoma tissue was lower than in the normal gastric mucosa tissue, with significant statistical difference（X^2 = 68.01 ,P 〈 0.05 ）. There were significant statistical differences between the positive expression rates of livin and Caspase-3 in the tissues of different clinical stages, histological differentiation, depth of tumor invasion and lymph node metastasis （ P 〈 0.05 ）. The positive expression rate of livin protein were negatively correlated to that of Caspase-3 protein in stomach adenocarcinoma tissue（ rs = -0.57, P 〈 0.05 ）. Conclusion The expression of livin and Caspase-3 in stomach adenocarcinoma tissue is negatively correlated, which indicates that livin may promote the occurrence and progression of stomach adenocarcinoma via inhibiting apoptosis.