HPLC-UV法测定草决明中五种蒽醌类化合物含量及其指纹图谱的建立

Determination of Five Anthraquinones and Establishment of a Fingerprint of Cassia Occidentalis Seeds by HPLC with UV Detection

目的:建立一种同时测定草决明中五种蒽醌类化合物含量的HPLC方法及草决明HPLC指纹图谱。方法:采用Hypersil C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.05%甲酸水溶液为流动相,梯度洗脱;流速1.0mL/min,柱温:30℃,检测波长254nm。采用评价软件(2004A)进行数据分析,对来自加纳和中国13个地区,共22种生、炒草决明建立了HPLC指纹图谱,并进行相似度计算。结果:方法的灵敏度、线性、精密度以及准确度均符合方法学验证的要求。分别在生品中检出16个共有峰,在炮制品检出8个共有峰;与对照样品(加纳的生品)相比,中国的两个样本相似系数分别为0.577(南京)和0.565(武汉)。结论:所建立的草决明指纹图谱的方法稳定可靠,适用于草决明质量评价,两个国家的草决明质量有明显差异。

Objectiev ： To establish a simultaneous determination of five cassia anthraquinones content by HPLC , and HPLC fingerprint method of grass cassia .M ethod：Hypersil C18 column （4.6 mm ×250 mm, 5μm）, acetonitrile -0.05%formic acid as a mobile phase .The flow rate was 1.0 mL/min, the temperature was 30℃, and the detection wave length was 254 nm.Using chromatographic fingerprint evaluation software （ 2004 A） for data analysis , from Ghana and China , established HPLC fingerprint and similarity calculation .Result： The sensitivity, linearity , precision and accuracy of the method are in line with the requirements of method validation .16 and 8 common peaks were detected in crude drug and processed one respec-tively,compared with the control sample （ Ghana raw goods ） , similar coefficients of two samples from China were 0.577 （ Nanjing ） and 0.565 （ Wuhan ） , indicating significant difference between two areas of grass Cassia in quality .Conclusion：Cassia HPLC fingerprint method established stable and reliable , and suitable for quality evaluation Cassia herbs .