闽产太子参核苷类成分积累动态的QTRAP LC-MS/MS分析

Dynamic changes of nucleosides and nucleobases in Pseudostellariae Radix from Fujian province analyzed by QTRAP LC-MS/MS

目的:分析不同生长期太子参中核苷类成分积累的动态变化。方法:采用QTRAP LC-MS/MS技术同时测定太子参样品中13种核苷类成分的含量。结果:太子参核苷类成分中,以胞苷、尿苷、肌苷、乌苷、胸苷含量较高;不同生长期太子参核苷类成分含量有所差异,7月份含量相对较高。结论:太子参核苷类成分的积累与生长期密切相关,其积累高峰期与当地传统采收期基本一致。

Objective： To analyze the dynamic changes of nucleosides and nucleobases in Pseudostellariae Radix at different growth periods. Methods： QTRAP LC-MS/MS method was applied for the analysis of 13 kinds of nucleosides and nucleobases in Pseudostellariae Radix. Results： The contents guanosine and thymidine were higher in Pseudostellariae Radix. The contents were ods; the contents were relatively higher in those grown in July. Conclusion： The nucleobases in Pseudostellariae Radix is closely related to its growth cycle and the of cytidine, uridine, inosine, different at different growth peri- accumulation of nucleosides and conventional collecting time.