摘要
为制备犬细小病毒特异性抗血清,用犬细小病毒DD株免疫健康易感比格犬,无菌采血后分离血清制备犬抗CPV-2血清。通过SN、ELISA、IFA、HI方法对制备的血清进行检测,未检出犬类常见的5种病毒抗体,说明该血清特异性良好。经测定,该血清中和抗体效价为1∶512;HI效价为1∶1280。应用结果表明,该血清对不同犬细小病毒毒株中和能力相当,对CPV-2感染犬有良好的治疗效果。
Sensitive canines were immunized with CPV DD strain for preparation anti-CPV-2 polyclonal antibody. The collected canine antisera were free of 5 kinds of foreign antibody by SN, ELISA, IFA or HI. The titers of the serum were 1 ∶ 512 by SN, 1 ∶ 1280 by HI. The studies showed that the antisera had the same neutralizing capality for different CPV-2 strains,and also may be used for treatment of CPV-2 canine.
出处
《中国兽药杂志》
2014年第10期11-13,共3页
Chinese Journal of Veterinary Drug
基金
科技部科技基础性工作专项(2008FY130100)
关键词
犬细小病毒
抗血清
制备
canine parvovirus
polyclonal antibody
preparation
