长春市市售猪肉鸡肉中金黄色葡萄球菌和沙门氏菌分离及耐药性分析

Isolation and Drug Resistance Analysis of S.aureus and Salmonella spp.from Retail Chicken and Pork in Changchun

对肉品中金黄色葡萄球菌及沙门氏菌进行耐药分析，以期为其风险评估提供依据。采集吉林省长春市市售猪肉、鸡肉样品，按照国标方法分离金黄色葡萄球菌、沙门氏菌，利用K-B法药敏试验检测金黄色葡萄球菌和沙门氏菌对头孢噻肟钠、左旋氧氟沙星、庆大霉素、四环素4种抗生素的耐药性。结果显示：共分离出金黄色葡萄球菌46株，检出率为46％；沙门氏菌48株，检出率为16．84％。金黄色葡萄球菌对4种抗生素的耐药率在0～56．52％之间，共产生6种耐药谱。沙门氏菌对4种抗生素的耐药率在16．67％～87．5％之间，共产生9种耐药谱。长春市肉品中金黄色葡萄球菌、沙门氏菌耐药现象普遍。

This study aimed at updating knowledge on the antimicrobial resistance characteristics of S.aureus and Salmonella spp. from chicken and pork in Changchun. The results collected during this study provided the first baseline data for risk assessment. Chicken and pork samples were collected in Changchun. S. aureus and Salmonella spp. were isolated according to National Food Safety Standard. The susceptibility to CEX,GEN,LVX and TET was performed according to the disk diffusion testing. Results showed that 46 S.aureus and 48 Salmonella spp. were separated from fresh chicken and pork. The resistance frequency of S. aureus to all antibiotics was 0~56.52%. 6 resistance profiles of S.aureus were found in this study. The resistance frequency of Salmonella spp. to all antibiotics was 16. 67%~87. 5%. There were 9 resistance profiles. Antibiotic resistance of S. aureus and Salmonella spp. was common in the isolates from chicken and pork in Changchun.