摘要
建立了7种兽药中非法添加对乙酰氨基酚、安乃近、地塞米松和地塞米松磷酸钠药物的HPLC-PDA法。采用十八烷基键合硅胶为填充剂，磷酸二氢钠缓冲液（磷酸二氢钠3．0 g加水至1000 mL，加三乙胺1 mL，用氢氧化钠调pH值至7．0±0．2）-甲醇（80∶20）为流动相，梯度洗脱，二极管阵列检测器进行全扫描和240 nm波长测定，并采用峰纯度检查和光谱相似度检查辅助对照品比对方法，对四种目标分析物进行确证。结果显示，4种解热镇痛抗炎药物与其他物质峰分离良好，在测定范围内线性关系良好，平均回收率为73．5％～119．2％，RSD为0．1％～5．8％。本方法准确、可靠、重现性好，可用于兽药制剂中非法添加四种解热镇痛抗炎药物的定性和定量检测。
A method was established for the determination of four kinds of illegal additives antipyretic-analgesic and anti-inflammatory drugs in veterinary drug by the high performance liquid chromatography with photo-diode array detector. It was tested with C18 column, using gradient elution with sodium dihydrogen phosphate buffer-methanol（80 ∶ 20,V/V） as the mobile phase. The extract wavelength was 240 nm from the full scan, peak purity test and spectral similarity were helped to identify the four kinds of target analytes. Results showed that four kinds of target analytes were finely separated, and all the compounds showed good linearity in the concentration range of detection. The mean recovery of four antipyretics were 73.5%~119.2%,RSD were 0.1%~5.8%. In conclusion, the method was accurate and reproducible, and provided a reliable way for detecting antipyretic- analgesic and anti-inflammatory drugs adulterated in veterinary drug.
出处
《中国兽药杂志》
2014年第10期36-43,共8页
Chinese Journal of Veterinary Drug
基金
河南省重点科技攻关计划(132102110078)
