高尔基体蛋白73和磷脂酰基醇蛋白聚糖-3联合测定在原发性肝癌早期诊断中的价值

Value of Golgi protein 73 combined with phosphatidyl alcohol proteoglycans-3 detection in early diagnosis of primary liver cancer

目的探讨高尔基体蛋白73(GP73)和磷脂酰基醇蛋白聚糖-3(GPC3)联合测定在原发性肝癌(PLC)早期诊断中的价值。方法选择40例PLC患者、48例肝硬化患者、45例肝炎患者及44例健康对照患者,采用酶联免疫法检测血清中的GP73及GPC3水平。结果肝癌组患者血清中GP73为(812.37±81.59)pg/mL,GPC3为(89.27±12.38)ng/mL,GPC3、GP73阳性表达率分别为82.5%、87.5%,均明显高于其他3组,肝硬化组与肝炎组GPC3、GP73表达水平、阳性表达率显著高于健康对照组;GPC3、GP73联合检测灵敏度、特异度、准确度分别为92.5%、100%、93.81%,显著高于GP73、GPC3单一检测(P<0.05)。结论 GP73与GPC3联合检测可显著提高PHC准确率,对PLC的早期诊断具有较高的价值。

Objective To explore the value of Golgi protein 73 （GP73 ）combined with phosphatidyl alcohol proteoglycans-3 （GPC3 ）detection in early diagnosis of primary liver cancer （PLC）.Methods The serum GP73 and GPC3 levels were detected in 40 PLC patients,48 patients with liver cirrhosis,45 hepatitis patients and 44 healthy people by ELISA.Results Serum GP73 was （812.37±81.59）pg/mL and GPC3 was（89.27±12.38）ng/mL in PLC group,and GPC3, GP73 positive expression rate were 82.5%,87.5% respectively,which were significantly higher than the other 3 groups.GPC3 and GP73 levels,positive expression rate in liver cirrhosis patients and hepatitis patients were significantly higher than healthy control group.Sensitivity,specific de-grees and accuracy of GPC3 combined with GP73 detection were 92.5%,100% and 93.81%, which were significantly higher than those of GP73 ,GPC3 single test （P＆lt;0 .05 ）.Conclusion GP73 combined with GPC3 detection can significantly improve the accuracy of PHC,which shows a high value in early diagnosis of PLC.