六味地黄颗粒对晚期肝肾阴虚型结直肠癌患者基因表达谱的差异分析

Difference analysis of Liuwei Dihuang Granule on the gene expression profile of patients with colorectal cancer due to yin deficiency of liver and kidney

目的筛选肝肾阴虚型晚期结直肠癌(CRC)患者使用六味地黄颗粒前后的基因表达谱差异,探索六味地黄颗粒药效的基因组学机制。方法选取晚期肝肾阴虚型CRC患者以六味地黄颗粒进行干预,21 d为1个疗程。干预期间患者不接受其他西医及中医药治疗。21 d后再次提取外周血,进行基因芯片检测,并与干预前芯片结果进行自身对照分析。结果与干预前相比,干预后基因芯片检测发现129个差异基因条,其中128个上调,1个下调。基因功能(GO)富集分析结果显示,干预前后共发现254个基因GO存在显著差异(P<0.05或P<0.01)。在生物过程中,凝血功能相关的基因占41.5%;在细胞组成中,45.5%的差异基因与细胞质膜有关;在分子功能方面,64.9%的差异基因与结合有关。结论晚期CRC肝肾阴虚型患者应用六味地黄颗粒干预前后存在差异表达基因,功能分析显示六味地黄颗粒可增强患者凝血功能,增加钙离子结合。

Objective The genomics mechanism of Liuwei Dihuang Granule efficacy was ex-plored by selecting the different gene expression profiles before and after the application of Liuwei Di-huang Granule for patients with advanced colorectal cancer （CRC）due to yin deficiency of liver and kidney.Methods Liuwei Dihuang Granule was given to patients with advanced CRC due to yin defi-ciency of liver and kidney,21 days as a course.During treatment,no western medicines or other Chi-nese medicines were administrated.21 days later,peripheral blood was collected again to detect the gene chips.Then the chips after intervention were compared with themselves before intervention.Re-sults Compared with intervention before,the chips after intervention had 129 differential gene frag-ments,in which 128 were up-regulated and 1 was down-regulated.Gene function enrichment anal-ysis showed that a total of 254 gene ontologies （GO）had significant differences before and after inter-ventions （P〈0.05 orP〈0.01）.In the biological process,coagulation-related genes accounted for 41.5%.In cellular components,45.5%differential cells was associated with plasma membrane while in molecular function,64.9% was correlated with combination.Conclusion Patients with advanced CRC due to yin deficiency of liver and kidney have differential gene expressions before and after the in-terventions of Liuwei Dihuang Granule,and GO analysis indicates that Liuwei Dihuang Granule can strengthen the coagulation function and combination with calcium of CRC patients.