SUZ12蛋白对口腔鳞状细胞癌的致癌作用

Role of SUZ12 in oral squamous cell carcinoma carcinogenesis

目的分析SUZ12蛋白的表达水平与口腔鳞状细胞癌(OSCC)患者恶性生物学行为的关系,并研究SUZ12蛋白在口腔鳞状细胞癌细胞系中的生物学功能。方法对2008至2013年哈尔滨医科大学附属第一医院96例口腔鳞状细胞癌患者石蜡标本通过免疫组化方法检测SUZ12蛋白的表达;在体外实验中,通过RNA干扰的手段抑制SUZ12蛋白在口腔鳞状细胞癌细胞系中的表达水平。结果 SUZ12蛋白在OSCC组织中表达增高,其增高与肿瘤的分化,淋巴结转移相关(P<0.001);此外,发现抑制SUZ12蛋白的表达抑制了口腔鳞状细胞癌细胞的增殖,促进了口腔鳞状细胞癌细胞的凋亡。结论SUZ12起到癌基因的作用,高表达的SUZ12蛋白水平在口腔鳞状细胞癌发生发展过程中起到重要作用。

Objective To examine a potential correlation of SUZ12 expression with clinicopathological parameters in patients with oral squamous cell carcinoma（ OSCC） and investigate the biological function of SUZ12 in OSCC cell line. Methods The SUZ12 expression levels were determined by performing immunohistochemical analysis in 96 patients with OSCC from the First Affiliated Hospital of Harbin Medical University,from 2008 to 2013. In vitro analysis,a stable SUZ12 knocking down cell line was generated with RNAi. Results High SUZ12 expression was found in OSCC tissue and this over-expression was associated with tumor differentiation,lymph node metastasis（ P 〈0. 001）,furthermore,knocking down of SUZ12 induced apoptosis and decreased the proliferation ability of OSCC cells. Conclusion SUZ12 acts as an oncogene and high SUZ12 expression promotes carcinogenesis in OSCC.