摘要
目的 探讨瑞舒伐他汀、氯沙坦以及二者联合对氧化修饰低密度脂蛋白(ox-LDL)诱导的人单核巨噬细胞小凹蛋白-1表达的影响.方法 分离培养人单核细胞并诱导转化为巨噬细胞,建立动脉粥样硬化模型,分别以不同剂量瑞舒伐他汀(0.1、1.0、5.0 μmol/L)、氯沙坦(10、50、100μmol/L)以及联合两药最适剂量(5.0 μmol/L+ 100 μmol/L)干预细胞,以RT-PCR检测各组小凹蛋白-1的mRNA表达.结果 与空白对照组比较,ox-LDL组小凹蛋白-1 mRNA表达显著减少[(0.2533±0.00973) vs (0.9410 ±0.03677)],其差异有统计学意义(P<0.01);与ox-LDL组比较,随着瑞舒伐他汀及氯沙坦浓度的升高,小凹蛋白-1 mRNA表达均逐渐升高[(0.5198±0.04840)、(0.6183±0.06740)、(0.7257±0.03052) vs(0.2533±0.00973);(0.3350 ±0.04177)、(0.4428 ±0.03804)、(0.6049 ±0.02627) vs (0.2533 ±0.00973)],各组间比较差异有统计学意义(P<0.05);最适剂量瑞舒伐他汀及氯沙坦联合组与单药组比较,小凹蛋白-1 mRNA表达显著升高,各组间比较差异均有统计学意义(F =59.119,P<0.01).结论 瑞舒伐他汀及氯沙坦均可以通过ox-LDL途径上调人单核巨噬细胞小凹蛋白-1 mRNA表达,且呈一定的剂量依赖趋势;两药以最适剂量联合应用后,小凹蛋白-1 mRNA表达较单药更为显著.
Objective To investigate the effects,mechanisms,and the optimum doses of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells which were induced by oxidized low density lipoprotein(ox-LDL).Methods Human-monocyte cells were separated and changed into the human monocyte-macrophage cells.The model of amerosclerosis was set up.These cells were incubated in different doses of Rosuvastatin(0.1,1.0,5.0 μmol/L) and Losartan (10,50,100 μmol/L),and then cultured in combination of two drags (5.0 μmol/L + 100 μmol/L).Expression of caveolin-1 mRNA was determined with real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Results In ox-LDL group,caveolin-1 mRNA was decreased sharply relative to control group [(0.2533 ±0.00973) vs (0.9410 ±0.03677)] in a concentration-dependent manner (P <0.01).Compared to ox-LDL group,expressions of Caveolin-1 mRNA were increased gradually in different doses of Rosuvastatin alone and Losartan alone group [(0.5198 ± 0.04840),(0.6183 ± 0.06740),(0.7257 ± 0.03052) vs (0.2533 ± 0.00973) ; (0.3350 ± 0.04177),(0.4428 ± 0.03804),(0.6049 ± 0.02627) vs (0.2533 ± 0.00973)] in a concentration-dependent manner (P < 0.01) ; the summit expressions of caveolin-1 mRNA were emerged in using Rosuvastatin and Losartan together (F =59.119,P < 0.01).Conclusions Rosuvastatin and Losartan may be responsible for the expression of caveolin-1 in human monocyte-macrophage cells that were induced by ox-LDL.The expressions were up-regulated with dose dependent manner of these drugs,and got the crest stage when using optimum doses of Rosuvastatin and Losartan together.
出处
《中国医师杂志》
CAS
2014年第8期1052-1055,共4页
Journal of Chinese Physician
基金
山西省科技攻关项目(20120313020-10)