粒细胞集落刺激因子对诱导成骨的作用

Granulocyte colony-stimulating factor induces osteogenesis

背景:间充质干细胞是一种具有自我复制能力和多向分化潜能的成体干细胞,在特定条件下能够发育成骨、软骨和其他类型的细胞,而粒细胞集落刺激因子可以促进间充质干细胞的再生。目的:通过动物实验来观察粒细胞集落刺激因子对成骨的诱导作用,以期对临床骨缺损的患者提供一种更好的治疗方法。方法:取新西兰白兔40只,随机等分为治疗组及模型组,均切除右侧约1 cm长度桡骨及周围骨膜,之后在缺损处植入脱钙骨基质。治疗组选用粒细胞集落刺激因子30μg/(kg·d)皮下注射,于造模前3 d开始,连续应用7 d;模型组给予生理盐水皮下注射。结果与结论:治疗组兔骨缺损区间充质细胞、成骨细胞数量和成骨质量上均优于模型组。提示粒细胞集落刺激因子的应用可以明显提高骨缺损区域的间充质干细胞数量,促进成骨,从而可以促进骨缺损的早期修复。

BACKGROUND：Mesenchymal stem cells are a kind of adult stem cells with the potentials of self-replicating and multi-directional differentiation. Under certain conditions, the cells can develop into bone, cartilage and other types of cells. Granulocyte colony-stimulating factors can promote regeneration of mesenchymal stem cells. OBJECTIVE：To observe the osteogenic induction effect of granulocyte colony-stimulating factors by animal experiment, and provide a better treatment method for clinical treatment of bone defect. METHODS：Forty New Zealand rabbits were randomly divided into treatment group and model group. Al animals were removed 2 cm length of the right radial bone and the surrounding periosteum, and then implanted with demineralized bone matrix into the defect. The treatment group was subcutaneously injected with granulocyte colony-stimulating factor 30μg/kg per day, the injection was started at preoperative 3 days and given for 7 continuous days. In control group, the same dose of physiological saline was injected. RESULTS AND CONCLUSION：In the treatment group, the number of mesenchymal stem cells, the quantity and quality of bone formation at bone defects were better than those in the model group. The experimental findings indicate that granulocyte colony-stimulating factor can obviously increase the number of mesenchymal stem cells and promote bone formation at the area of bone defect, which can contribute to early repair of bone defects.