化学去细胞尿道在大鼠皮下的埋置

Subcutaneous embedment of the acellular urethra in rats

背景:有研究提出了移植排斥反应的实质是受者免疫系统对供者移植物抗原的免疫应答反应。目的:验证化学萃取去细胞处理对同种异体尿道移植抗原性的影响。方法:取SD大鼠尿道共20根,每段长1 cm。其中10根采用化学去细胞法处理大鼠尿道的免疫原性成分,使其成为去细胞尿道;另外10根不做去细胞处理为新鲜尿道。在10只Wistar大鼠背部皮下植入去细胞尿道,另取10只大鼠背部皮下植入新鲜同种异体尿道。6周后取材行大体和组织学检查。结果与结论:20只Wistar大鼠在皮下埋植尿道后均无自嗜肢体现象,切口愈合良好,饮食正常,无溃疡发生。6周后取材大体观察可见去细胞尿道周围有组织膜形成,而新鲜尿道未见组织膜形成。苏木精-伊红染色可见去细胞尿道逐渐被吸收,被平滑肌组织取代,并可见大小不等血管长入去细胞尿道内,部分有形成血窦的倾向;对照组尿道未见吸收,未见血管长入。提示化学去细胞尿道在大鼠体内逐渐形成类似正常尿道的组织材料,说明化学去细胞同种异体尿道移植的抗原性明显降低,可替代正常尿道海绵体组织。

BACKGROUND：The nature of graft rejective reaction is the antigen immunological reaction of recipient’s immune system in response to donor’s graft. OBJECTIVE：To observe the effect of chemical extraction on the immunogenicity of acellular peripheral urethral isografts. METHODS：Twenty urethras of Sprague-Dawley rats, 1 cm long, were included in this study. Ten urethras were treated with chemical acellular method to remove immunogenic component, thus prepared into acellular urethras;another ten urethras were fresh, without any acellular treatment. Acellular urethras were implanted into the back of ten Wistar rats, while fresh urethras into another ten rats. 6 weeks after transplantation, the specimens were harvested for gross and histological observations. RESULTS AND CONCLUSION：After acellular or fresh urethras were implanted into the rats, no autotomy was observed, the incision healed wel , the diet and activities of the rat were normal, and no ulcer formation was found. At 6 weeks, tissue membrane formed around the acellular urethra, while no formation was visible around fresh urethra by gross observation. Hematoxylin-eosin staining showed that, acellular urethras were gradual y absorbed and replaced by smooth muscular tissue. And unequal blood vessels grew in the acellular urethra, even formed blood sinusoid. In the control group, the urethras were not absorbed and no blood vessels were grown. The acellular urethras can gradual y form normal urethra in rats, indicating that the acellular urethral autograft has a lower antigenicity and can replace the cavernous body of normal urethra.