冬凌草甲素对人肺癌NCI-H460细胞侵袭和迁移的影响

Effect of oridonin on invasion and migration of human lung cancer NCIH460 cells

目的:研究冬凌草甲素对人肺癌NCI-H460细胞侵袭和迁移的影响。方法:将NCI-H460细胞分为高剂量(HD)组、中剂量(MD)组、低剂量(LD)组和对照(N)组,前3组(实验组)加入不同浓度的冬凌草甲素,N组不加冬凌草甲素,观察细胞生长情况;用MTT法测定细胞增殖;比较细胞侵袭能力和迁移能力;Western blotting检测细胞MMP-2和MMP-9表达。结果:实验组细胞数量低于对照组,细胞增殖受抑制,HD组抑制率为48.94%,MD组为36.17%,LD组为19.15%;实验组穿透滤膜细胞数明显减少,HD组为26.67±5.16,MD组为36.17±5.08,LD组为44.33±5.50;实验组的迁移速率及迁移细胞数均降低;实验组的MMP-2和MMP-9表达水平明显低于N组(P<0.01)。结论:冬凌草甲素能抑制NCI-H460肺癌细胞的侵袭和迁移,其作用与下调MMP-2和MMP-9表达有关。

AIM：ToinvestigatetheeffectoforidoninontheinvasionandmigrationofhumanlungcancerNCI-H460 cells.METHODS：NCI-H460 cells were divided into high-dose （HD）, middle-dose （MD） and low-dose （LD） oridonin groups （cultured with 40, 20 and 10μmol/L of oridonin, respectively, as experimental groups）, and normal （N） group （ treated without oridonin as control ） .The cell growth was observed .The cell proliferation was detected by MTT as-say.Boyden chamber was used to determine the cell invasive capacity .The cell migration was also measured .The levels of MMP-2 and MMP-9 were assayed by Western blotting .RESULTS：The cell counts in the experimental groups were lower than that in N group .The cell proliferation was inhibited as the inhibitory rates were 48.94%, 36.17%and 19.15% for HD group, MD group and LD group, respectively.The numbers of the invasive cells were 26.67 ＆#177;5.16 for HD group, 36.17 ＆#177;5.08 for MD group, and 44.33 ＆#177;5.50 for LD group.The migration rates in the experimental groups were lower than that in N group .The expression of MMP-2 and MMP-9 decreased dependent on the oridonin dose as follows： HD group ＆lt;MD group ＆lt;LD group ＆lt;N group.CONCLUSION：Oridonin inhibits the invasion and migration of NCI-H460 lung cancer cells, and reduces the expression of MMP-2 and MMP-9.