羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性

In vitro cytotoxicity of the hydroxyapatite/barium titanate biological piezoelectric ceramic coating

背景:为优化羟基磷灰石的生物活性,前期实验利用等离子喷涂技术在羟基磷灰石表面等离子喷涂制备了压电陶瓷涂层,但此种新型材料的细胞毒性还不明确。目的:评价羟基磷灰石/钛酸钡压电陶瓷涂层的体外细胞毒性。方法:将第3代比格犬骨髓间充质干细胞分别接种于羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件上,接种5 d后,扫描电镜观察材料上细胞黏附情况。分别以羟基磷灰石/钛酸钡压电陶瓷试件浸提液、羟基磷灰石试件浸提液、含5%二甲基亚砜和体积分数15%胎牛血清的低糖DMEM溶液、只含体积分数15%胎牛血清的低糖DMEM培养基培养第3代比格犬骨髓间充质干细胞,培养的1,3,5 d采用CCK-8法检测各组细胞毒性。结果与结论:骨髓间充质干细胞在羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件表面增殖旺盛,达到复层生长,伪足与细胞之间连接很紧密,胞体丰富,表明两组材料具有良好的细胞相容性。CCK-8细胞毒性实验显示,羟基磷灰石/钛酸钡压电陶瓷试件与羟基磷灰石试件组细胞相对增殖率均在80%以上,毒性分级为1级,无细胞毒性。

BACKGROUND：In order to optimize the biological activity of hydroxyapatite, previous experiments have used plasma spraying technique to prepare a piezoelectric ceramic coating on the surface of hydroxyapatite, but the cytotoxicity of this new material is not clear. OBJECTIVE： To evaluate the cytotoxicity of hydroxyapatite/barium titanate biological piezoelectric ceramic coatingin vitro. METHODS： The 3rd generation beagle bone marrow mesenchymal stem cels were seeded on hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens, respectively. After 5 days, the celladhesion was detected by scanning electron microscopy. The 3rd generation bone marrow mesenchymal stem cels were also co-cultured with hydroxyapatite/barium titanate piezoelectric ceramic specimen extract, hydroxyapatite specimen extract, low-glucose Dulbecco’s modified Eagle’s medium containing 5% dimethylsulfoxide and 15% fetal bovine serum, and low-glucose Dulbecco’s modified Eagle’s medium containing 15% fetal bovine serum, respectively. The cytotoxicity was tested by cellCounting Kit-8 assay at days 1, 3, 5 after co-culture. RESULTS AND CONCLUSION：Bone marrow mesenchymal stem cels on the surface of hydroxyapatite/barium titanate piezoelectric ceramic specimens and hydroxyapatite specimens grew proliferatively and presented with multi-layer growth. The connection between cels and pseudopodia was very close, which indicates that the two kinds of materials both have good cytocompatibility. cellCounting Kit-8 assay showed that the cels cultured in the extracts of hydroxyapatite/barium titanate biological piezoelectric ceramic and hydroxyapatite specimens proliferated more than 80%, and the toxicity was grade 1 that meant no cytotoxicity.