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模拟失重条件下大鼠心室肌Beclin-1和LC3的表达变化 被引量：2

Effects of Simulated Weightlessness on Expression of Beclin-1 and LC3 in the Ventricular Muscle of Rats
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摘要 目的探讨模拟失重大鼠心室肌自噬相关基因Beclin-1和微管相关蛋白轻链3(LC3)的表达变化。方法雄性SD大鼠12只,随机分为对照组和模拟失重组。模拟失重4周后取心室肌组织,采用RT-PCR和Western Blot方法测定Beclin-1和LC3 mRNA及蛋白表达的变化。结果与对照组相比,模拟失重4周后大鼠心室肌Beclin-1和LC3 mRNA及蛋白表达均显著增强(P<0.05)。结论模拟失重可诱导大鼠心室肌自噬相关基因Beclin-1和LC3的高表达,提示模拟失重可能引起了心室肌自噬的激活。 Objective To explore the expression of autophagy related gene Beclin-1 and LC3 in the ventricular muscle after simulated weightlessness. Methods A total of 12 male SD rats were randomly assigned to control and simulated weightlessness groups. The ventricular muscle tissues of rats subjected to simulated weightlessness were taken after 4 weeks of simulated weightlessness,respectively. RT-PCR and Western Blot were applied to determine the expression of autophagy related gene Beclin-1 and LC3. Results Compared with the control group,the expression of Beclin-1 and LC3 mRNA and protein in the ventricular muscle were both significantly increased after 4 weeks of simulated weightlessness. Conclusion Simulated weightlessness increases the expression of autophagy related gene Beclin-1 and LC3,suggesting autophagy may be induced by simulated weightlessness in the ventricular muscle of rats.
作者 赵疆东 王永春 李小涛 陈荣贵 孙喜庆
机构地区 第四军医大学航空航天医学系航空航天生物动力学教研室 西安电子科技大学体育部
出处 《航天医学与医学工程》 CAS CSCD 北大核心 2014年第4期291-294,共4页 Space Medicine & Medical Engineering
基金 国家自然科学基金资助项目(81171872和81372130)
关键词 模拟失重 心室肌 自噬 基因表达 simulated weightlessness ventricular muscle autophagy gene expression
分类号 R854 [医药卫生—航空、航天与航海医学]
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参考文献16

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航天医学与医学工程
2014年 第4期

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