可溶性PD-1在原发性肝癌患者外周血的表达及其临床意义

Expression and clinical significance of sPD-1in peripheral blood of patients with HBV-related primary hepatic carcinoma

目的探讨可溶性程序性死亡1(sPD-1)在原发性肝癌(PHC)患者外周血中的表达及其临床意义。方法采用ELISA法检测60例PHC患者(PHC组)、30例乙肝肝硬化患者(肝硬化组)、30例慢性乙型肝炎患者(慢乙肝组)和20例健康人(对照组)外周血中sPD-1的表达水平;采用流式细胞术检测患者的外周血CD8+T淋巴细胞上PD-1的表达。结果 PHC组外周血sPD-1表达水平为(0.55±0.15)ng/ml,低于肝硬化组的(1.91±1.16)ng/ml、慢乙肝组的(2.55±1.40)ng/ml和对照组的(4.03±0.86)ng/ml(P<0.05);而PHC组患者外周血CD8+T淋巴细胞PD-1的表达水平为(28.8±2.3)%,高于肝硬化组的(21.4±1.9)%,慢乙肝组的(16.3±2.1)%和对照组的(11.0±1.7)%(P<0.05)。PHC患者外周血sPD-1表达水平与肝癌临床分期和Child-Pugh分级密切相关(P<0.05)。结论 PHC发生可能与sPD-1表达水平下降致免疫功能下降有关。

Objective To explore the expression and clinical significance of soluble programmed death-1（sPD-1）in the peripheral blood of patients with HBV-related primary hepatic carcinoma（PHC）.Methods With ELISA and flnuorescence-activated cell sorterer techniques,the expressions of sPD-1and PD-l on CD8＋T lymphocytes from periipheral blood were detected in the patients with PHC（group A,60cases）,patients with hepatitis cirrhosis（group B,30cases）,patients with chronic hepatitis B（group C,30cases）,and healthy people（group D,20cases）.Results Serum level of sPD-1in group A was（0.55±0.15）ng/ml,which was significantly lower than（1.91±1.16）ng/ml in group B,（2.55±1.40）ng/ml in group C,and（4.03±0.86）ng/ml in group D（P0.05）.The PD-1level on CD8＋T lymphocytes in the patients with PHC was（28.8±2.3）%,which was significantly higher than（21.4±1.9）% in group B,（16.3±2.1）% in group C and（11.0±1.7）% in group D（P0.05）.The sPD-1expression in group A was closely related with clinical stages and Child-Pugh grades（P0.05）.Conclusion The development of PHC may be related with a decreased immune function induced by low expression of sPD-1.