摘要
【目的】比较稻瘟病抗性基因Pi-km的不同分子标记,筛选能真实反映其表型的分子标记用于辅助育种选择。【方法】本研究利用与稻瘟病抗性基因Pi-km紧密连锁的1个SNP标记及Pi-km基因内部的2个Indel标记,对62份2012年长江上游国家水稻区域试验材料和97份陕西省水稻区试材料进行了基因型分析,并结合这些材料的稻瘟病抗性的田间鉴定结果,分析了基因型与抗病性之间的关系,对不同的分子标记辅助育种选择进行了评价。【结果】2类标记检测的基因型不完全一致,基于Pi-km基因序列设计的标记比与其连锁的标记作为辅助育种选择更准确。【结论】Pi-km基因的稻瘟病抗性与基因型有关,基因型为M1-M2-的材料比其他基因型材料抗病性趋势更强。
[Objective] The different molecular markers of resistant gene Pi-km of rice blast were compared. Selecting the markers that can represent true phenotype was helpful for breeding. [Method] The gene Pi-km was a main resistant allele of Pik locus to rice blast. The genotypes of Pi-km in 62 rice trials in 2012 in the upper Yangtze river and 97 rice trials in Shan＇xi were analyzed using one SNP marker that was linked to Pi-km and two Indel markers within Pi-km. In combina- tion with field data of resistance of those lines to rice blast, the relationship between genotypes of those markers in Pi-km and disease resistance was evaluated. [Results] The results showed that genotyping results were not completely consistent between SNP and Indel markers. Indel markers within Pi-km were more accurate than the SNP marker that tightly linked to Pi-km in marker-assisted selection. [Conclusion] Rice blast resistance was associated with the genotypes identified by the markers. The resistance of lines with M1-M2-genotype was stronger than that of other genotypes.
出处
《四川农业大学学报》
CSCD
北大核心
2014年第3期252-259,共8页
Journal of Sichuan Agricultural University
基金
陕西省科技厅农业科技攻关项目(2013K02-10-01)
陕西省科技厅农业科技创新项目(NKC01-01)
