筛选产纤维素酶菌株及其产酶条件的优化

Screening of Cellulose Producer Strains and Optimization of the Conditions for Cellulose Production

[目的]筛选降解菌糠纤维素的菌株,并且优化其发酵条件。[方法]采用纤维素-刚果红培养基进行筛选,筛选出纤维素酶活较高的菌株。通过比较不同氮源、碳氮比例等条件下CMC酶活,研究其最佳发酵条件。[结果]选出一株酶活较高的菌株,命名为N3。其最适有机氮源为豆饼粉,无机氮源是(NH4)2SO4。最适合N3产酶的(NH4)2SO4∶豆饼粉比例为2∶4。最适碳源氮源比例为5∶2。[结论]N3是一株具有研究价值的产纤维素酶的菌株。

[ Objective ] To screen strains producing cellulose and optimize the culture conditions. [ Method ] With Cellulose Gango Red Medium in screening, a high-yield cellulose strain was obtained. The culture conditions were optimized by comparing the CMCase on the kinds and ration of nitrogen source. [ Result] N3 was isolated. The highest level of cellulose activity was induced in the medium containing organic nitrogenous （ soybean cake） and inorganic nitrogen （ ammonium sulfate） with the ration of 4： 2. The ratio of C to N is 5： 2. [ Conclusion ] N3 had a highly research value.