舒芬太尼后处理对缺血/再灌注大鼠心肌线粒体Cx43蛋白表达的影响

Effects of sufentanil post-conditioning on Cx43 in myocardial ischemia reperfusion of rats

目的观察舒芬太尼后处理对缺血/再灌注大鼠心肌线粒体Cx43蛋白表达的影响。方法将60只SD大鼠随机分为A、B、C、D组各15只,B组结扎大鼠左冠状动脉前降支(LAD)30 min,松开结扎线灌注120 min,制作心肌缺血/再灌注模型;A组只穿线,不结扎LAD,持续150 min;C组缺血30 min末行缺血30 s,再灌注30 s,重复3次,再灌注120 min;D组缺血30 min、再灌注前5 min静滴舒芬太尼0.1μg/kg,再灌注120 min。再灌注末取大鼠心脏,TTC法染色心肌切片并计算心肌梗死面积百分比(MIS%);Western blotting半定量法测定心肌线粒体总Cx43(tCx43)和磷酸化Cx43(pCx43)表达。结果 A、B、C、D组的MIS%分别为0.8%±2.4%、44.6%±5.3%、37.4%±3.7%、26.5%±4.2%,D组与B、C组相比,P均<0.05。以B组心肌线粒体tCx43和pCx43电泳条带灰度为基准,A、B、C、D组心肌线粒体tCx43灰度比分别为2.3、1.0、1.3、1.8;pCx43灰度比分别为2.4、1.0、1.3、1.6。B组有少量tCx43和pCx43蛋白表达;D组tCx43和pCx43蛋白表达明显高于B、C组(P均<0.05)。结论舒芬太尼可能通过增加缺血/再灌注大鼠心肌线粒体Cx43蛋白表达发挥心肌保护作用。

Objective To investigate the impaction of sufentanil post-conditioning on Cx43 expression of myocardial ischemia reperfusion injury （ I/RI） .Methods Sixty Sprague Dawley（ SD） rats were randomly divided into 4 groups （ n=15）.The group B：Ligature the left anterior descending coronary artery （LAD） of rat heart for 30 min of ischemia, then 120 min of reperfusion, the myocardial ischemia-reperfusion injury model could be obtained; the group A： only thread, without ligation of LAD for 150 min;the group C：ischemia 30 min line at the end of the 30 s, reperfusion 30 s, repeat 3 times, 120 min of reperfusion;the group D：5 min before 30 min of ischemia reperfusion intravenous drip sufentanil 0.1μg/kg, 120 min of reperfusion.Took out rat heart at the end of reperfusion, then calculated the percentage of infarct size of myocardium （ MIS%） by triphenyltet tetrazolium chloride （ TTC） staining, and observed the content of total connexin 43 （tCx43） and phosphorylated connexin 43 （pCx43） in myocardium by semiquantitative Western blotting.Results The MIS%of group A, B, C, D were respectively 0.8%±2.4%, 44.6%±5.3%, 37.4%±3.7%, 26.5%±4.2%, com-pared with group B and group C, MIS% in the group D was lower significantly, P〈0.05.Basing on the electrophoresis banding density of tCx43 and pCx43 of group B, the percentage of density of tCx43 of group A, B, C, D were respectively 2.3, 1.0, 1.3, 1.8;the percentage of density of pCx43 of group A, B, C, D were respectively 2.4, 1.0, 1.3, 1.6. There was little expression of tCx43 and pCx43 in group B;the expression of tCx43 and pCx43 in group D was higher signif-icatively than that in group B and group C （P〈0.05）.Conclusion Sufentanil may play a protective role on rat myocardi-al ischemia reperfusion injury by increasing the content of Cx43 of rat myocardium after the myocardial ischemia reperfu-sion.