基于线粒体COI基因部分序列的长江口虾虎鱼科鱼类系统分类

System classification for Gobiidae derived from the Yangtze River based on mitochondrial COI gene partial sequence

对采自长江口的7种虾虎鱼(Gobiidae)19个个体的线粒体COI基因进行测序,其他6种虾虎鱼的相应序列从Genbank中获得。序列分析结果显示7种虾虎鱼的COI基因部分序列密码子的第一位碱基的GC碱基含量最恒定,第三位碱基的GC含量最高,平均A+T的含量要高于G+C的含量,与多种鱼类的COI基因的碱基特点相似。运用MEGA5.0计算13种虾虎鱼的种间遗传距离,表明拉氏狼牙虾虎鱼(Oxyurichthy tentacularis)与孔虾虎鱼(Chaeturichthys stigmatias)归属于近盲虾虎鱼亚科,根据种间遗传距离与系统发育树的结构特征推测,触角沟虾虎鱼和矛尾虾虎鱼可能具有共同的起源。

A total of nineteen specimens of seven species of Gobiidae derived from the Yangtze River were couected to se -quence mitochondrial COI genes.Sequences from homologous regions of six other species of this family were gathered from Genbank.The results showed that the most constant GC content was in the first base of codon and the highest GC content existed in the third base of codon .The average content of A ＋T was higher than the average content of G ＋C.The feature was similar to most fishes .Comparing the COI gene of thirteen species of Gobiidae with MEGA 5.0 , the taxonomic status of Odontamblyopus lacepedii and Trypauchen vagina were proved legitimately .The genetic distance between species and the structure feature of Phylogeny tree indicated the same origin of Oxyurichthy tentacularis and Chaeturichthys stigmatias.