不同换药方式对糖尿病模型小鼠和正常小鼠创面愈合影响的实验研究

Experimental study on influence of different dressing changes on wound healing in diabetic model mice and normal mice

[目的]比较生理盐水、4%硼酸溶液、0.5%碘伏+3%过氧化氢3种临床常用护理换药方式对糖尿病小鼠和正常小鼠创面愈合的影响,以期探索最适合糖尿病难愈性创面的护理换药方式。[方法]取雄性8周龄C57BL/6J小鼠30只,设糖尿病组与对照组小鼠各15只,糖尿病小鼠以链脲佐菌素(STZ诱导)在背部制作3个同等大小的全层皮肤创面后,每日1次分别以生理盐水(A处理)、4%硼酸溶液(B处理)和0.5%碘伏+3%过氧化氢溶液(C处理)对创面换药,记录创面结痂及感染情况并计算创面愈合率。[结果]在同一时相点、A处理换药方式下,糖尿病组小鼠的创面愈合率明显低于对照组小鼠;对照组小鼠在3种不同换药处理方式下,其创面愈合率差异无统计学意义(P>0.05);糖尿病小鼠应用B处理的创面愈合率显著高于应用A和C处理法(P<0.05)。[结论]正常小鼠创面应用3种换药方式对创面的愈合率无显著影响;糖尿病小鼠创面使用硼酸溶液换药较其他换药方式更有利于伤口愈合。

Objective：To compare the influence of 3clinical common nursing dressing methods including physiological saline,4% boric acid solution,and 0.5%iodophor＋3%hydrogen peroxide on wound healing in diabetic mice and normal mice,so as to explore the most suitable nursing dressing changes for diabetic refractory wound.Methods：A total of30 male C57BL/6Jmouse at age of 8weeks were taken and divided into diabetic group and control group,15 cases in each.The streptozotocin（STZ induced）was placed on the back of diabetic mouse to make 3equal sized full-thickness skin wounds in them,and then the normal saline（A treatment）,4%boric acid solution（B treatment）and 0.5%iodophor＋3%hydrogen peroxide solution（C treatment）were used respectively for dressing changes of three wounds once a day.The wound scab and infection were recorded and the wound healing rate was calculated.Results：At the same time point,in A treatment wound healing rate of diabetic mice in diabetic group was significantly lower than that in control group;there was not statistically significant difference in wound healing rate of control group mouse among 3different dressing treatment（P〈0.05）;the wound healing rate in diabetic mouse using B treatment was significantly higher than that of A and C treatments（P〈0.05）.Conclusion：3kinds of dressing changes applied for wounds in normal mice have no significant influence on wound healing rate;boric acid solution used for wounds dressing changes in diabetic mice is more conducive to wound healing compared with other dressing changes.