山东产忍冬不同药用部位金属元素及总黄酮类化学成分的比较研究

Comparison Study on Metal Elements and Total Flavonoids Contents in Different Medicinal Parts of Flos Lonicerae in Shandong

目的研究山东产忍冬不同药用部位的微量金属元素及黄酮类化学成分含量及其组成差异。方法采用ICP-AES法(电感耦合等离子体发射光谱法)测定锌、铁、锰、钙4种元素的含量,比色法测定总黄酮含量。结果金银花植株藤、叶、花蕾等不同器官均含有金属元素及总黄酮,忍冬植物花蕾中的总黄酮含量最高,达3.50%;其次是忍冬茎,为1.99%;叶中的总黄酮含量最低,仅1.60%。叶中的锌、铁两种元素的含量最高,花蕾与藤中锌、铁元素含量次之,彼此相差较小。藤与叶中的锰、钙元素含量相差较小,远超过花蕾中的含量。结论山东产忍冬不同部位皆含有微量元素及总黄酮,且具有一定的差异。从药食同源的角度,忍冬的应用部位可进一步拓展,同时,总黄酮含量结果与忍冬药用部位不断演变,花部药用逐步受到重视的情况是相符的。

Objective To study the contents of metal elements and total flavonoids in different medicinal parts of Flos Lonicerae in Shandong, and compare the difference of their chemical constituents. Methods ICP-AES was established to determine the contents of Zn, Fe, Mn, Ca, and colorimetric method was adopted for the determination of total flavonoids. Results The stem, leaf, flower of Flos Lonicerae all contained metal elements and total flavonoids. The content of total flavonoids was highest in the bud, up to 3.50 %, followed by 1.99 % in the stem. The content of total flavonoids in leaves was lowest, only 1.60 %. The contents of Zn and Fe were highest in the leaves and lower in the bud and stem with a little difference. The Ca and Mn contents in the leaves and stem were similar, and were much higher than that of the bud. Conclusion Different parts of Shandong Flos Lonicerae contain trace elements and total flavonoids, and the contents have certain difference. From the perspective of homology of medicine and food, the application parts of Flos Lonicerae can be further expanded. At the same time, the determination results of total flavonoids in Flos Lonicerae can be consistent with the evolving medicinal parts and gradually increasing attention to floral medicinal material.