甘蓝型油菜TFⅢA型锌指蛋白基因鉴别和表达模式

Identification and expression patterns of TFⅢA- type zinc finger genes in Brassica napus

植物TFⅢA型锌指蛋白的锌指区含有保守的QALGGH区,属于C2H2型转录因子,参与调控植物生长发育和逆境胁迫应答.参照拟南芥AtZFP基因的C2H2结构域氨基酸序列,采用生物信息学方法鉴定了46个甘蓝型油菜TFⅢA型锌指蛋白基因,分析了基因结构、生化特性、进化关系和基因表达模式.结果表明：42个基因为单锌指蛋白基因,4个为多锌指蛋白基因;40个基因不含内含子,6个基因含内含子;46个基因的氨基酸序列同源性变幅为9.0% ～99.2%,氨基酸序列保守性差异明显,亚类成员间包含相同的保守基序,亚类间的保守基序不同;PlantCARE分析发现其启动子区富含激素、非生物胁迫及特定生理过程响应的顺式作用元件;RNA-seq分析表明,部分基因在甘蓝型油菜品种中双11号的根、初花期雌蕊和角果中特异表达.

Plant TF ⅢA- type zinc finger proteins harboring a plant- specific conservative QALGGH sequence known as classical C2H2- type transcription factors,play crucial roles in development regulation and environmental stress response. To investigate the homologs in Brassica napus,46 TFⅢA- type zinc finger genes in B.napus were identified through amino acid sequence alignment of AtZFP against Brassica databases. Among the 46TFⅢA- type zinc finger genes,42 contain mono zinc finger domain and 4 harbor multiple zinc finger domains; in addition,6 out of these 46 genes have introns with in. An obvious amino acid homology variation were found ranging from 9. 0% to 99. 2%. Phylogenetic analysis indicated that the motif was highly conserved within each. Promoter prediction revealed that there were abundant cis- acting elements responding to hormone,abiotic stress and specific physiological processes within the promoter regions. Tissue- specific expression patterns of these TFⅢA-type zinc finger genes were detectable among RNA- seq datasets of 12 tissues( siliques,roots and initial pistils,etc.) of an elite B. napus cultivar Zhongshuang 11.