16S rDNA克隆文库法分析地下水生物反硝化系统细菌种群多样性

Bacteria Diversity in a Denitrification-based Groundwater Remediation System by 16S rDNA

采集地下水硝酸盐生物反硝化系统内的污泥样品,提取污泥样品中微生物的总DNA,构建细菌16S rDNA基因片段克隆文库,并通过16S rDNA序列系统发育分析,对反硝化系统内的细菌种群多样性以及菌群结构进行了研究。结果表明,地下水生物反硝化系统内细菌具有高度多样性,样品文库分为9个细菌类群,优势菌群为β-Proteobacteria(67.11%)和Bacteroidetes(15.79%),其中,β-proteobacteria为最优势菌群,以Rhodocyclaceae为主。对反硝化系统内细菌种群多样性的研究有利于确定优势菌种,为地下水硝酸盐生物反硝化修复奠定理论基础。

Sludge sample was collected from a denitrification-based groundwater remediation system.Bacterial genome DNA was extracted,and then a bacterial 16 S rDNA genetic library was built up.After the phylogenetic analysis of 16 S rDNA sequences,bacterial community structure and diversity of the denitrification-based groundwater remediation system were studied.The results indicated that the bacterial community in the denitrification-based groundwater remediation system was highly diverse,and could be divided into nine major groups.The dominant bacterial communities in the denitrification-based groundwater remediation system were β-Proteobacteria and Bacteroidetes,which accounted for 67.11% and 15.79% of the total clones,respectively.β-Proteobacteria predominant in the ecosystem,and especially Rhodocyclaceae has absolute advantage in β-Proteobacteria clone library.The study of bacterial diversity of the denitrification-based groundwater remediation system will help to ascertain the dominant bacterial community and established a theoretical foundation for denitrification-based groundwater remediation.