利用F_(2:3)和BC_2F_2群体定位水稻粒型和粒重QTL

Mapping QTLs for grain shape and weight using F_(2:3) and BC_2F_2 population in rice

以两个粳稻品种东农425和长白10为亲本,建立分别含有180、190个株系的F2:3和BC2F2群体,构建两张含有137个SSR标记的遗传图谱,对水稻粒长、粒宽、粒厚、长宽比和千粒重5个性状进行QTL定位分析。结果表明,检测到分布于水稻1、2、3、5、7、8、10、11和12号染色体上,粒长15个、粒宽8个、粒厚6个、长宽比11个、千粒重10个,共50个QTL位点。其中6个QTL qGL10、qGW3-2、qGT3-2、qL/W1-2、qTGW5和qTGW8-3的贡献率较大,为控制各性状的主效QTL。两群体定位结果相互比较,在RM1235、RM1352和RM1285标记处为粒型和粒重QTL分布的热点区域。

Total 180 F2：3 population lines and 190 BC2F2 population lines derived from a cross between japonica rice Dongnong425 and Changbai10,and their both linkage map including 137 SSR markers were used to map QTL control ing grain shape（grain length, grain width, grain thickness and ratio of grain length to width） and thousand-grain weight traits. The results showed that total 50 QTLs control ing the five grain traits were detected on chronosome 1, 2, 3, 5, 7, 8, 10, 11 and 12, respectively, including 15 QTLs for grain length, eight QTLs for grain width, six QTLs for grain thickness, 11 QTLs for ratio of grain length to width and 10 QTLs for thousand-grain weight. Six QTLs named qGL10, qGW3-2, qGT3-2, qL/W1-2, qTGW5 and qTGW8-3 could explain much of the observed phenotypic variance, and they were major QTLs control ing related traits. Compared the mapping QTLs results in both population, the marker named RM1235, RM1352 and RM1285 were QTLs for grain shape and weight distribution hotspots.