GLi-1和SFRP1在浸润性乳腺癌中的表达及意义

The significance of GLi-1 and SFRP1 expression in invasive breast carcinoma

目的:探讨锌指转录因子1(Glioma-associated oncogene homolog-1,GLi-1)和分泌型卷曲相关蛋白1(Secreted Frizzled-Related Proteins-1,SFRP1)在浸润性乳腺癌中的表达及相关性。方法:采用免疫组化PV-9000二步法检测68例浸润性乳腺癌和24例癌旁乳腺组织中GLi-1和SFRP1表达。结果:浸润性乳腺癌组GLi-1阳性率显著高于癌旁乳腺组(P<0.05);浸润性乳腺癌组SFRP1阳性率显著低于癌旁乳腺组(P<0.05);随着浸润性乳腺癌TNM分期的增加,GLi-1阳性率增高,SFRP1阳性率降低,差异均有显著性意义(P<0.05);GLi-1和SFRP1在浸润性乳腺癌中的表达呈负相关。结论:Gli-1与SFRP1涉及Hh和Wnt通路的异常调控,在浸润性乳腺癌中的表达存在负相关关系,且与肿瘤TNM分期有关,其作用机制值得进一步研究。

Objective： To investigate the expression and correlation of Glioma-associated oncogene homolog1（GLi-1） and secretedFrizzled-Related Proteins1（SFRP1） in invasive breast carcinoma. Methods： The expression of GLi-1, SFRP1 was detected in 68 invasivebreast carcinomas tissues and 24 adjacent normal breast tissues by PV two-step immunohistochemical method. Results： The results showedthat the positive rates of GLi-1 in invasive breast carcinoma were significantly higher than those in adjacent normal breast tissues （P〈0.05）;the positive rates of SFRP1 in invasive breast carcinoma were significantly lower than these in adjacent normal breast tissues （P〈0.05）;with the increase of TNM stage of invasive breast carcinomas, the positive rates of GLi-1 increased, the positive rates of SFRP1 decreased,the differences were both significant （P〈0.05）. The expression of GLi-1 and SFRP1 showed a significantly negative correlation （P〈0.05）,with the increase of GLi-1 protein expression, the SFRP1 expression decreased. Conclusion： The expression of Gli-1 and SFRP1 ininvasive breast carcinoma had a negative correlation and associated with tumor TNM stage, which is related to the abnormal regulation ofHh and Wnt pathway. The mechanism of this is deserve further research.