党参黄酮提取部位对小肠上皮细胞迁移及多胺的影响

Effect of Radix Codonopsis Flavonoids on Intestinal Endothelial Cells 6 Migration and Polyamines Content

目的观察党参黄酮提取部位对小肠上皮细胞(IEC-6)迁移及迁移过程细胞内多胺(精脒)含量的影响。方法划痕法复制细胞迁移模型,观察在多胺合成抑制剂二氟甲基鸟氨酸(DFMO)及钾通道抑制剂4-氨基吡啶(4-AP)负荷下,党参黄酮提取部位对细胞迁移的影响;柱前衍生-高效液相色谱法检测给予党参黄酮提取部位12 h和24 h后细胞内精脒的含量。结果党参黄酮提取部位可促进IEC-6细胞迁移,逆转DFMO或4-AP所致细胞迁移抑制;党参黄酮提取部位给药12 h和24 h后可提高细胞内精脒含量。结论党参黄酮提取部位促进细胞迁移的作用与影响多胺调控信号通路有关。

Objective To investigate the effect of Radix Codonopsis flavonoids on intestinal endothelial cells 6 （IEC-6） migration and the content of intracellular polyamine（spermidine） during cell migration. Methods Cell migration model was established by scratch test, and then was used for investigating the effect of Radix Codonopsis flavonoids on cell migration, which was inhibited by difluorometylornithine （DFMO, an irreversible specific inhibitor of polyamines） and 4-amino pyridine （4-AP, a potassium inhibitor）. After coculture with the flavonoids for 12 and 24 hours, the content of intraeellular polyamine （spermindine） in IEC-6 was detected by pre-column derivatization high performance liquid chromatography（HPLC ） method. Results Radix Codonopsis flavonoids promoted IEC-6 migration, reversed the cell migration inhibition induced by DFMO or 4-AP. After administration of Radix Codonopsis flavonoids for 12 and 24 hours, the the content of intracellular spermidine was increased. Coclusion The effect of Radix Codonopsis flavonoids on promoting IEC-6 migration may be related to its regulation of polyamine-dependent signaling pathway.