芍苓汤对豚鼠心得安模型及HaCaT细胞炎症因子IL-6、IL-17A和IL-22mRNA表达的影响

Effect of Shaoling Decoction on Propranolol-induced Psoriasis-like Model of Guinea Pigs and mRNA Expression of IL-6,IL-17A and IL-22 in HaCaT Keratinocyte Cell Line Stimulated by Lipopolyasccharide

目的研究芍苓汤对心得安豚鼠模型的影响及对脂多糖(LPS)诱导人永久分化表皮角质细胞(HaCaT细胞)产生炎症因子IL-6、IL-17A和IL-22 mRNA表达的调控作用。方法以Baker评分法观测芍苓汤对豚鼠心得安模型的作用,实时荧光定量PCR(RT-Q-PCR)检测芍苓汤对LPS诱导HaCaT细胞IL-6、IL-17和IL-22 mRNA表达水平。结果芍苓汤能够显著改善心得安诱发豚鼠的银屑病样病变,并显著下调LPS诱导的IL-6 mRNA表达。结论芍苓汤具有治疗银屑病的药效,其作用机制与下调IL-6 mRNA表达水平有关。

Objective To observe the effect of Shaoling Decoction （SLD） on guinea pigs propranolol-induced psoriasis- like model and to investigate its regulatory effect on mRNA expression of IL-6, IL-17A and IL-22 in HaCaT keratinocyte cell line stimulated by ipopolyaseeharide （LPS）. Methods Baker scoring method was used for the evaluation of Shaoling Decoction on the guinea pigs propranolol-induced psoriasis-like model, and real time fluorescence quantitative PCR （RT-Q-PCR） was applied for the detection of mRNA expression of IL-6, IL-17A and IL-22 in HaCaT line stimulated by LPS. Results Shaoling Decoction had an obvious effect on ameliorating the psoriasis-like pathological changes in guinea pigs, and on down-regulating IL-6 mRNA expression in LPS-activated HaCaT cell line. Conclusion Shaoling Decoction has certain therapeutic effect for psoriasis, and its mechanism may be related with down-regulation of IL-6 mRNA expression.