胃疡宁丸体内外抗幽门螺杆菌的实验研究

Antimicrobial Activities of Weiyangning Pills Against Helicobacter Pylori in Vivo and in Vitro

目的研究胃疡宁丸在体内、体外抗幽门螺杆菌(Helicobacter pylori,HP)的作用机制。方法采用液体稀释法测定胃疡宁丸和阿莫西林对HP的最小抑菌浓度(Minimum Inhibitory Concentration,MIC)。将BALB/c小鼠随机分为6组,即正常对照组、模型对照组、阿莫西林组(0.4mg·g-1)及胃疡宁丸低、中、高剂量组(0.79,1.58,3.16 g·kg-1·d-1)。采用抗生素加HP的方法,连续灌胃7 d,建立HP感染动物模型。从第11天开始,连续灌胃给药8周后处死小鼠,取胃黏膜和血清进行快速尿素酶测试、血清HP抗体检测以及病理切片检查。结果液体稀释法测得胃疡宁丸最小MIC值为0.0416 g·mL-1;阿莫西林最小MIC值为0.00012 g·mL-1。快速尿素酶测试、血清幽门螺杆菌抗体检测、小鼠胃黏膜病理切片结果均提示HP感染动物模型复制成功,与正常对照组比较,差异有显著性意义(P<0.05,P<0.01)。阿莫西林、胃疡宁丸高、中、低剂量组均能有效抑制体内HP的生长,与模型对照组比较,差异有显著性意义(P<0.05,P<0.01)。结论胃疡宁丸在体内和体外均能有效地抑制幽门螺旋杆菌的生长。

Objective To study the antimicrobial activities of Weiyangning Pills（WP） against helicobacter pylori in vivo and in vitro. Methods By using broth dilution method, we determined the minimum inhibitory concentration （MIC） of WP and Amoxicillin. The Balb/c mice were randomized divided into normal control group, model group, Amoxicillin （0.4 mg ·g^-1）, and low-, middle- and high-dose WP groups. The mice were inoculated orally with antibiotic on first day and with Helicobacter pylori strains for 7 continuous days to establish HP infection model. From experimental day 11, the mice were given oral administration of the corresponding drugs for 8 weeks. At the end of the experiment, gastric mucosa and serum were obtained for rapid urase test （RUT）, serum HP antibody test, and pathological section examination. Results The MIC of WP was confirmed as 0.0416 g·mL^-1 and that of amoxicillin was 0.00012 g·mL^-1. The result of RUT, serum antibody test and pathological section examination showed that HP infection model had been established successfully, and both amoxicillin and WP inhibited the growth of HP in vivo（compared with the normal control,P 〈 0.05 or P 〈 0.01 ）. Conclusion Weiyangning Pills are effective on inhibiting the growth of HP in vivo and in vitro.