北京市食源性金黄色葡萄球菌耐药及分子分型研究

Study of antimicrobial resistance and molecular characteristics in foodborne Staphylococcus aureus in Beijing

目的分析北京市食源性金黄色葡萄球菌对常用抗生素的耐药情况及其分子分型特征,预防和控制由该菌引起的食源性疾病,为临床合理用药提供依据。方法收集2010—2012年北京地区食源性致病菌监测网分离到的金黄色葡萄球菌187株,采用微量肉汤稀释法检测金黄色葡萄球菌对8种常用抗生素的敏感性。采用脉冲场凝胶电泳(PFGE)进行分子分型。结果 116株金黄色葡萄球菌表现出对抗生素耐药,耐药率为62.03%,对抗生素的耐药率为红霉素(ERY)45.45%、苯唑西林(OXA)27.81%、克林霉素(CLI)20.86%、四环素(TET)13.90%、氯霉素(CHL)11.76%、环丙沙星(CIP)6.42%、甲氧苄啶/磺胺甲恶唑(TPM/SMZ)5.88%。其中耐甲氧西林金黄色葡萄球菌(MRSA)52株、对甲氧西林敏感的金黄色葡萄球菌(MSSA)135株。分离到的MRSA中34株菌表现出多重耐药。全部菌株共分为129种PFGE带型,优势带型不明显。结论食源性金黄色葡萄球菌的耐药和多重耐药普遍存在,PFGE带型分布分散与耐药谱的关联性较差。

Objective To analyze the antimicrobial resistance and molecular characteristics in foodborne Staphylococcus aureus in Beijing so as to prevent foodborne disease caused by Staphylococcus aureus and provide evidence for the reasonable clinical use of antibiotics. Methods Staphylococcus aureus strains isolated from foodborne pathogenic bacteria monitoring network in Beijing from 2010 to 2012 were tested against 8 commonly used antibiotics using broth micro-dilution method. The isolates were further subtyped by PFGE. Results 116 strains were antibiotic resistant, the resistant rate was 62.03%. The resistant rates for 8 commonly used antibiotics were erythromycin （45.45%）, oxacillin （27. 81% ）, clindamycin （20.86%）, tetracycline （13.90%）, chloramphenicol （11.76%）, ciprofloxacin （6.42%）, trimethoprim/sulfamet- hoxazole （5.88%）. 52 strains were Methicillin-resistant Staphylococcus aureus （MRSA）, and 135 strains were methicillinsensitive Staphylococcus aureus （MSSA）. 34 MRSA were multidrug resistant. Totally, 129 PFGE patterns were identified. No dominant PFGE patterns were obviously identified. Conclusion Muhidrug resistant were popular among the Staphylococcus aureus strains isolated from food. PFGE patterns showed diversed characteristics. The antibiotic resistance spectrum and PFGE patterns showed no correlation.