摘要
目的建立食源性金黄色葡萄球菌的分型与溯源方法。方法以血浆凝固酶为目标基因,通过PCR方法对食源性金黄色葡萄球菌进行鉴定,同时借助全自动微生物基因指纹鉴定系统(RiboPrinter)对其进行分型和类聚状况分析。结果 PCR方法可特异性鉴定金黄色葡萄球菌,RiboPrinter方法可将金黄色葡萄球菌分为6个亚型,各亚型类聚状况平均分布。结论上述方法的建立可准确地将金黄色葡萄球菌进行鉴定和分型,分析类聚分布与同源性关系,为食源性致病菌的快速鉴定与分型溯源提供技术手段。
Objective To develop a genotyping and tracing method for foodborne pathogenic Staphylococcus aureus （S. aureus）. Methods Foodborne S. aureus isolates were identified through PCR method targeting coagulase gene and subtyped by RiboPrinter. Results 62 isolates were identified as S. aureus and classified into six subtypes, and each subtype was hypodispersion. Conclusion Cooperative use of PCR method and RiboPrinter method could precisely identify and classify S. aureus contamination, analyze the distribution and characteristic, and trace the possible fingerprint. This research may provide experimental data for pathogenic bacteria typing, and give technical support for food supervision and administration.
出处
《中国食品卫生杂志》
北大核心
2014年第5期475-480,共6页
Chinese Journal of Food Hygiene
