溃疡性结肠炎体内Toll样受体5核因子-κB及白细胞介素17表达及相关性研究

Expression and significance of toll-like receptor 5,NF-kappaB and interleukin 17 in patients with ulcerative colitis

目的探讨Toll样受体5(TLR5)、核因子-κB(NF-κB)及白介素17(IL-17)在溃疡性结肠炎(UC)患者结肠组织及外周血中的表达及临床意义。方法收集2012年12月至2014年1月郑州大学第一附属医院门诊和住院的活动期UC患者70例,其中轻度UC患者30例,中度25例,重度15例。另外收集30名健康志愿者的血清样本作对照,20例结肠癌行外科手术切除的正常结肠断端取正常结肠组织作对照。分别采用免疫组织化学SABC检测结肠黏膜中TLR5与NF-κB的表达,ELISA检测外周血IL-17的含量。结果 TLR5和NF-κB在各组结肠黏膜的表达差异及IL-17在各组外周血的表达差异均有统计学意义(P<0.01),UC患者组高于对照组,重度UC患者高于中度及轻度UC患者。另外,TLR5与IL-17呈显著正相关(r=0.823,P<0.01)。结论 TLR5可能通过活化NF-κB增加IL-17的表达参与了溃疡性结肠炎的发生与发展,检测其变化对疾病严重程度的评价具有重要临床意义。

Objective To explore the expression of Toll-like receptor 5 （ TLR5 ）, NF-KappaB （NF-κB） and Interleukin-17 （IL-17） and analyse their relationship in patients＇ colon tissue and peripheral blood with ulcerative colitis （UC）. Methods A total of 70 initially active UC patients were collected, among which 30 patients were mild UC ,25 patients were moderate and 15 patients were severe UC. Their peripheral blood and colon mucosa were collected. The colon mucosa tissues from 15 cancer patients were collected as tissue control, blood samples from 30 healthy volunteers were collected as serum control. BThe expression of TLR5 and NF-κB in the colon mucosa were measured by immunohistochemistry, and the level of IL-17 in serum were detected by ELISA. Results As compared with control group,the TLR5 ,NF-KB and IL-17 in UC group were significantly increased （P 〈 O. 01 ）. And the severe and moderate group were significantly higher than mild group. What＇s more, the expression of TLR5 were positively correlated with NF-κB and IL-17 （ P 〈 0. O1 ）. Conclusion TLR5 can induce activation of trans6ription factor NF-κB to enhance the expression of IL-17, all of which is involved with the the occurrence and development of UC.