金胺“O”荧光染色法在痰液结核杆菌检测中的临床应用

Application of Auramine "O" fluorescent staining for detection on Mycobacterium tuberculosis in sputum

目的:探讨荧光染色法在检测痰中结核杆菌中应用效果。方法:选择疑似结核病患者90例。每名患者收集即时痰、清晨痰和午夜痰3份痰标本,每份痰标本分别采用金胺"O"荧光染色法和抗酸染色法进行结核杆菌检测,比较两种方法的检测结果。结果:两种方法检测的阳性例数比较差异无统计学意义(P>0.05)。270份痰标本中检测荧光染色法检测结果为1+的标本21份,抗酸染色13份,荧光染色的显著高于抗酸染色(P<0.05)。不同时间痰标本两种方法检测阳性率没有显著差异(P>0.05)。结论:金胺"O"荧光染色法用于痰中结核杆菌的检测具有简便、检出率高等优点。

Objective： To discuss the application of Auramine ＂O＂ fluorescent staining for detection on Mycobacterium tuberculosis insputum. Methods： Ninety cases suspected tuberculosis were selected. Instant sputum, early morning sputum and midnight sputum werecollected from every patient. Every sample was detected by Auramine ＂O＂ fluorescent staining and acid-fast staining, and results of twomethods were compared. Results： Positive cases of two methods showed no significant difference（P〉0.05）. 1＋ samples detected byAuramine ＂O＂ fluorescent staining were 21, by acid-fast staining were 13, which were lower than Auramine ＂O＂ fluorescent staining（P〈0.05）. The positive rate of sputum samples at different times showed no significant difference between two methods（P〉0.05）. Conclusion：Auramine ＂O＂ fluorescent staining for detection on Mycobacterium tuberculosis in sputum has the advantages of simple application andhigh detection rate.