大承气汤降低心脏骤停后综合征兔血清类胰蛋白酶及炎性细胞因子的水平

Dachengqi decoction reduces the serum levels of mast cell tryptase and inflammatory cytokines in rabbits with post-cardiac arrest syndrome

目的观察大承气汤对心脏骤停后综合征(PCAS)兔血清肥大细胞类胰蛋白酶和单核细胞趋化蛋白-1(MCP-1)、白细胞介素8(IL-8)水平的影响。方法大耳白兔30只,随机分为假手术组、模型组、大承气汤组。采用窒息性心脏骤停制备兔PCAS模型。大承气汤组在自主循环恢复后15 min给予大承气汤每日15 g/kg灌胃(分2次使用)。分别在0、24、48、72 h观察反映器官功能的检测指标,采用ELISA测定兔血清类胰蛋白酶、MCP-1、IL-8的水平。结果大承气汤可明显减轻心、脑、肝、肾功能障碍。PCAS模型组血清类胰蛋白酶、MCP-1和IL-8含量均明显高于假手术组(P<0.01)。大承气汤组血清类胰蛋白酶含量(6 h)、MCP-1含量(6、24、48 h)和IL-8含量(6、24 h)均明显低于PCAS模型组(P<0.05或P<0.01)。结论大承气汤可降低PCAS兔血清类胰蛋白酶、MCP-1、IL-8的水平。

Objective To observe the effects of Dachengqi decoction on serum levels of mast cell tryptase, monocyte chemoattractant protein-1 （MCP-I） and interleukin-8 （IL-8） in rabbits with post-cardiac arrest syndrome （PCAS）. Methods Thirty healthy male Japanese rabbits were randomly divided into three groups： sham-operation group, PCAS model group and Dachengqi decoction treatment group. The model of PCAS was established by asphyxia-induced cardiac arrest. Fifteen minutes after return of spontaneous circulation, Dachengqi decoction [15 g/（kg · d）, bid] was given by intra-gastric adrrinistration in Dachengqi decoction treatment group. The indicators of organ function were evaluated 24, 48 and 72 hours after cardiac arrest. The serum levels of mast cell tryptase, MCP-1 and IL-8 were determined by ELISA. Results Dachengqi decoction alleviated the dysfunction significantly in heart, brain, liver and kidney. Compared with the sham group, the serum levels of mast cell tryptase, MCP-1 and IL-8 increased significantly in PCAS group （P 〈 0.01）. Compared with the PCAS group, the serum levels of mast cell tryptase （at 6 hours）, MCP-1 （at 6, 24 and 48 hours） and IL-8 （at 6 and 24 hours） decreased significantly in Dachengqi decoction treatment group （ P 〈 0.05 or P 〈 0.01 ）. Conclusion Dachengqi decoction can reduce the serum levels of mast cell tryptase, MCP-1 and IL-8 in rabbits with post-cardiac arrest syndrome.