超支化聚合物新材料富集糖肽方法的研究

Glycopeptides Extraction Using Hyperpolymer-assisted Hydrazide Functionalized Microsparticles

目的:将自合成的超支化聚合物固定到氨基硅球上形成一种新型的糖肽富集材料,用于糖肽的高效分离与富集。方法:阴离子开环聚合法合成的超支化聚缩水甘油,经高碘酸氧化成为超支化聚甘油醛(hyperbranched polyglycerol-aldehydes,HPG-ALD)后固定于氨基硅球上,再用己二酸二酰肼修饰得到的酰肼材料应用于糖肽富集。结果:经扫描电镜(Scanning Electron Microscope,SEM)、热重分析(thermogravimetric analysis,TGA)等手段证明材料被成功合成。同时,使用制备成功的新材料来富集标准糖蛋白和糖肽都获得了良好的富集效果。结论:实验证明超支化缩水甘油酰肼材料(hyperpolymer-assisted hydrazide functionalized microspheres,HHMs)可用于低丰度糖蛋白/糖肽的富集。

Objective： A novel approach was developed to selective extraction of glycoproteins by hyperpolymer-assisted hydrazide functionalized microsparticles（ HHMs）. Methods： Firstly, synthesizing hyperbranched poly-glycerols（ HPG） by ring-opening multibranching polymerization of glycidol using dioxane as an emulsifying agent following converted them to aldehyde-functional hyperbranched polyglycerol（ HPG-ALD）.Upon immobilization on the microspheres,aldehyde group of HPG-ALD were convert to hydrazide group via a reductive alkylation reaction. Conclusion： After characterized by scanning electron microscope（ SEM） and Thermo Gravimetric Analysis（ TGA）,it can be confirmed that HHMs were successfully synthesized and effectively used for the low-abundance glycoproteins / glycopeptides enrichment.